Epidermal barrier and keratinocyte differentiation: from normal skin to skin inflammatory diseases

Coordinator : M. SIMON

Scientific objectives


 

Terminal differentiation of the epidermis is an oriented process during which keratinocytes sequentially turn on and off a series of specific genes in the course of their migration to the external surface of the skin, through the spinous and granular layers. The ultimate step, or cornification, is a genuine process of programmed cell death which results in dramatic structural changes and degradation of the nucleus and cellular organelles, leading to the formation of corneocytes. These cells form the outermost cornified layer of the epidermis. Both the granular and cornified layers allow the epidermis to perform its vital function of multiple barrier between the individual and his environment through their involvement in innate immunity, their high mechanical strength, and their ability to detoxify reactive oxygen species, to limit water loss, to reduce the penetration of UV radiation and to prevent the infiltration of allergens and microorganisms.

From several decades, the objective of our team is to decipher, at the molecular level, the keratinocyte terminal differentiation program, and to know how it is impacted by the environment, and how its impairment induces skin and hair conditions. Thus our work is a continuum between basic research and translational studies. We particularly focus on ichthyoses and inflammatory diseases, including psoriasis and atopic dermatitis. In industrialized countries, the latter affect up to 20% of children and 10% of adults. They therefore represent a huge burden on health care.

 

 

Overview of our research

Our Projects


Molecular mechanisms of lamellar body biogenesis and secretion.

The epidermal barrier functions rely on the secretion by granular keratinocytes of the contents of tubulo-vesicular cytoplasmic structures related to lysosomes, derived from the Golgi apparatus, and called lamellar bodies. Lamellar bodies contain various enzymes, including lipases and proteases, anti-microbial peptides, corneodesmosin and lipids. Our aim is to decipher the molecular mechanisms involved in lamellar body biogenesis, trafficking and delivery.

        

In particular, we investigate the role of Rab family GTPases, cytoskeleton components, as well as the related signaling pathways. In previous reports, we have shown that the Rab11A GTPase and the Myosin5B actin-dependent motor are crucial for lamellar body biogenesis and trafficking. In our ongoing work, we take profit of a unique in vitro experimental model, 3D reconstructed human epidermis where gene modulation can be performed:  either down regulation by using RNA interference or overexpression of mutant sequences by using vectors allowing granular keratinocyte-specific expression. Our biological read-out is currently based on biochemical studies, photonic and electronic imaging, and functional assays.

 

 

 

 

 

 

Coordinator: Dr Corinne Leprince

Lipid metabolism and epidermal barrier: from the identification of mutated genes in Autosomal Recessive Congenital Ichthyoses to the patients’ treatment.

Inherited ichthyoses are rare monogenic diseases (prevalence 13.3 per million people in Europe) that occur at birth. They cause abnormal thickening of the skin, dryness, scaling, redness, itching and painful skin fissures involving life-long disfigurement and social ostracism. All forms of ichthyosis lead to a defective epidermal barrier. Recent researches on genes causing Autosomal Recessive Congenital Ichthyoses (ARCI) highlighted the essential role of lipids from the stratum corneum, in particular ceramides, in the epidermal permeability barrier.

Our aim is to better understand the molecular basis of this lipid-based permeability barrier in healthy skin and its impairment in ichthyoses. We are studying enzymes involved in the metabolism of skin ceramides and hope to decipher their subcellular localization and regulation. We particularly focus on PNPLA1 and LIPN, encoded by two ARCI-causing genes.

  

 

 

 

 

 

Another part of our goal is the pre-clinical development of targeted therapies to treat ichthyoses. The topical replacement of the missing lipid is a promising approach to improve the defective permeability barrier encountered in ARCI. We are developing, in an international multidisciplinary collaboration with chemists and experts in skin lipids, innovative substitution systems based on skin-identical lipids encapsulated in nanostructured formulations that will be validated for rescuing the epidermal barrier using pre-clinical in vitro and animal models of ichthyosis.

We also closely collaborate with dermatologists from the South Reference Center for Rare Skin and Mucous Membrane Diseases (recruitment of patients, clinical expertise in ichthyosis) and biologists (molecular diagnosis) at the Toulouse University Hospital. We have access to a large collection of biological samples from patients with ichthyosis and perform clinical and genetic studies to expand the knowledge of the molecular bases of ARCI and help to better define the patient’s characteristics. We also aim to identify undescribed ARCI-causing genes and potentially novel actors involved in lipid metabolism by combine approaches using next generation sequencing and bio clinical analyses.

 

To achieve all these projects, we benefit from our longstanding experience in the analysis of the epidermal barrier at the functional and molecular levels and we are currently developing high-performance organotypic models of ichthyoses by genome editing and 3D-culture of human keratinocytes.

Coordinator: Dr Nathalie Jonca

Atopic Dermatitis: pathophysiology, epidermal barrier dysfunctions and treatments.

Atopic Dermatitis (AD) pathogenesis results from complex interactions between genetic, immunological and environmental factors. But the primary defect that drives this type 2 immune disease remains controversial. A major discovery has been that loss-of-function mutations in FLG gene are the strongest known genetic risk factor for AD. Indeed, FLG encodes filaggrin, an epidermal protein specifically expressed by differentiated keratinocytes, and essential for the epidermal barrier function. This strongly indicates that skin barrier defects play a key role in the disease, enhancing the penetration of allergens and microbes through the epidermis and systemic IgE sensitization. Our aim is to better understand the relationship between the epidermal barrierdysfunction and the environmental factors in AD, and how this may help to identify new treatments. More specifically:

1) Using biochemical in vitro assays, reconstructed human epidermis, and mouse models, we focus on the enzymes (proteases, peptidyl-arginine deiminases (PADs), etc.) involved in the complex metabolism of filaggrin and of other proteins of the S100-fused type protein family, some of them being also associated with epidermal barrier dysfunction in AD. We study how keratinocytes adapt their filaggrin metabolism to the external environment, in particular the relative humidity level.

 

 

 

2) Pr Marie-Christine Cadiergues from the Dermatology Department of the National Veterinary School of Toulouse and Michel Simon detail the canine AD at the molecular level, since they believe that the dog disease may be a good model for the human one. Indeed, human and canine AD share many clinical characteristics, in particular spontaneous occurrence. They also develop canine skin models, to confirm pathophysiological hypotheses and test therapeutics, both for veterinary and human medicine.

 

 

3) In collaboration with the Ophthalmology and Dermatology Departments of Toulouse Hospital and the National Reference Center for Keratoconus, we have the opportunity i) to investigate, combining transcriptomic analysis and clinical characterization, why the development of severe conjunctivitis is the major adverse effect of Dupilumab, an anti-IL4/13 receptors used to treat AD patients; and ii) to test the hypothesis that corneal epithelium defects are involved in Keratoconus pathogenesis (a rare disease whose frequency is, for unknown reason, strongly enhanced in AD patients) and not only a consequence of stromal deformation.

 

4) In a multi-disciplinary (chemists, cell biologists and immunobiologists) collaborative work with R Poupot (Infinity Institute) and two other CNRS teams in Toulouse, we test the possibility to use original anti-inflammatory dendrimers as drugs for topical treatment of inflammatory skin diseases including severe forms of AD and psoriasis.

Coordinator: Dr Michel Simon

 

Other information


Publications

2022

Tatiana; He Lupasco, Zhiguo; Cassagne

Corneal epithelium in keratoconus underexpresses active NRF2 and a subset of oxidative stress-related genes. Journal Article

In: PLoS One, 2022.

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Aurélie; Clement Marches, Emily; Albérola

Cold Atmospheric Plasma Jet Treatment Improves Human Keratinocyte Migration and Wound Closure Capacity without Causing Cellular Oxidative Stress. Journal Article

In: Int J Mol Sci, 2022.

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Avinash; Srivastava Padhi, Ankit; Ramesh

IL-22 downregulates peptidylarginine deiminase-1 in human keratinocytes: adding another piece to the IL-22 puzzle in epidermal barrier formation. Journal Article

In: Invest Dermatol, 2022.

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Mariona; Bernard Rabionet, Pauline; Pichery

Epidermal 1-O-acylceramides appear with the establishment of the water permeability barrier in mice and are produced by maturating keratinocytes. Journal Article

In: Lipids, 2022.

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Buket; Cesarato Basmanav, Nicole; Kumar

Assessment of the Genetic Spectrum of Uncombable Hair Syndrome in a Cohort of 107 Individuals. Journal Article

In: JAMA Dermatol, 2022.

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Sébastien; Gault Cadau, Manon; Berthelemy

An Inflamed and Infected Reconstructed Human Epidermis to Study Atopic Dermatitis and Skin Care Ingredients. Journal Article

In: Int J Mol Sci. , 2022.

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Sara; Marches Gouarderes, Aurélie; Vicendo

Cold helium plasma jet does not stimulate collagen remodeling in a 3D human dermal substitute. Journal Article

In: Bio electrochemistry, 2022.

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Elodie; Abdallah Henriet, Florence; Laurent

Targeting TGF-β1/miR-21 pathway in keratinocytes reveals protective effects of silymarin on imiquimod-induced psoriasis mouse model. Journal Article

In: J Invest Dermatol Innov, 2022.

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Mandy; Clabbers Joosten, Julia; Jonca

New developments in the molecular treatment of ichthyosis: review of the literature. Journal Article

In: Orphanet J Rare Dis, 2022.

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Anna; Cassagne Kychygina, Myriam; Tauber

Dupilumab-Associated Adverse Events During Treatment of Allergic Diseases. Journal Article

In: Clin Rev Allergy Immunol. , 2022.

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Verena; Leprince Moosbrugger-Martinz, Corinne; Méchin

Revisiting the Roles of Filaggrin in Atopic Dermatitis. Journal Article

In: Int J Mol Sci. , 2022.

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Line-Alice; Combarros Lecru, Daniel; Moog

Multiplex Cytokine Analyses in Ear Canals of Dogs Suggest Involvement of IL-8 Chemokine in Atopic Otitis and Otodectic Mange-Preliminary Results. Journal Article

In: Animals (Basel). , 2022.

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Vincent; Mosca Bruet, Marion; Briand

Clinical Guidelines for the Use of Antipruritic Drugs in the Control of the Most Frequent Pruritic Skin Diseases in Dogs. Journal Article

In: Vet Sci. , 2022.

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Audrey; Bellon Lasek, Nathalie; Mallet

Effectiveness and safety of dupilumab in the treatment of atopic dermatitis in children (6-11 years): data from a French multicentre retrospective cohort in daily practice. Journal Article

In: J Eur Acad Dermatol Venereol., 2022.

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Eloi; Planells Debourdeau, Gabriel; Chamard

New Keratoconus Risk Factors: A Cross-Sectional Case-Control Study. Journal Article

In: J Ophthalmol. , 2022.

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Marie-Noelle; Bensefa-Colas Crépy, Linda; Aubin; of Allergology; French Society of Pediatrics, Venereology; French Society; of Occupational Medicine;, French Society

Vocational Guidance for Young Patients with Atopic Dermatitis: A Survey of Physicians' Opinions and Practices. Journal Article

In: Acta Derm Venereol. , 2022.

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2021

Sara; Marches Gouarderes, Aurélie; Vicendo

Cold atmospheric helium plasma does not stimulate dermal collagen remodeling at tissue scale. Journal Article

In: Bioelectrochemistry, 2021.

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Thomas; Amagai Luger, Masayuki; Dreno

Atopic dermatitis: Role of the skin barrier, environment, microbiome, and therapeutic agents. Journal Article

In: Journal of Dermatological Science, 2021.

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Daniel; Goudounèche Combarros, Dominique; Cadiergues

The upper epidermis of atopic dogs is altered at the functional and structural levels. Journal Article

In: Vet Dermatol, 2021.

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Alrun; Kopp Hotz, Julia; Bourrat

Meta-Analysis of Mutations in ALOX12B or ALOXE3 Identified in a Large Cohort of 224 Patients. Journal Article

In: Genes (Basel), 2021.

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Charline; Ravailhe Pressanti, Élodie; Castellote-Brun

Survey of cytokines on ocular surfaces of atopic dogs by multiplex analysis using two sampling methods - a pilot study. Journal Article

In: Vet Dermatol, 2021.

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2020

Tauber, M; Bérard, E; Lourari, S; Questel, E; Redoules, D; Paul, C; Simon, M

Latent class analysis categorizes chronic hand eczema patients according to skin barrier impairment. Journal Article

In: Journal of the European Academy of Dermatology and Venereology : JEADV, vol. 34, no. 7, pp. 1529–1535, 2020, ISSN: 1468-3083 (Electronic).

Abstract | Links | BibTeX

Touhouche, A T; Cassagne, M; Bérard, E; Giordano-Labadie, F; Didier, A; Fournié, P; Paul, C; Tauber, M

Incidence and risk factors for dupilumab associated ocular adverse events: a real-life prospective study. Journal Article

In: Journal of the European Academy of Dermatology and Venereology : JEADV, 2020, ISSN: 1468-3083 (Electronic).

Abstract | Links | BibTeX

Thyssen, Jacob P; Jakasa, Ivone; Riethmüller, Christoph; Schön, Michael P; Braun, Andrea; Haftek, Marek; Fallon, Padraic G; Wróblewski, Jacek; Jakubowski, Hieronim; Eckhart, Leopold; Declercq, Wim; Koppes, Sjors; Engebretsen, Kristiane A; Bonefeld, Charlotte; Irvine, Alan D; Keita-Alassane, Sokhna; Simon, Michel; Kawasaki, Hiroshi; Kubo, Akiharu; Amagai, Masayuki; Matsui, Takeshi; Kezic, Sanja

Filaggrin Expression and Processing Deficiencies Impair Corneocyte Surface Texture and Stiffness in Mice. Journal Article

In: The Journal of investigative dermatology, vol. 140, no. 3, pp. 615–623.e5, 2020, ISSN: 1523-1747 (Electronic).

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Tauber, Marie; Lourari, Siham; Bérard, Emilie; Questel, Emmanuel; Redoules, Daniel; Giordano-Labadie, Françoise; Simon, Michel; Carle, Paul

Positive change in hand care habits using therapeutic patient education in chronic hand eczema. Journal Article

In: Contact dermatitis, vol. 82, no. 1, pp. 10–17, 2020, ISSN: 1600-0536 (Electronic).

Abstract | Links | BibTeX

Méchin, Marie-Claire; Takahara, Hidenari; Simon, Michel

Deimination and Peptidylarginine Deiminases in Skin Physiology and Diseases. Journal Article

In: International journal of molecular sciences, vol. 21, no. 2, 2020, ISSN: 1422-0067 (Electronic).

Abstract | Links | BibTeX

2019

Cau, Laura; Takahara, Hidenari; Thompson, Paul R; Serre, Guy; Méchin, Marie-Claire; Simon, Michel

Peptidylarginine Deiminase Inhibitor Cl-Amidine Attenuates Cornification and Interferes with the Regulation of Autophagy in Reconstructed Human Epidermis. Journal Article

In: The Journal of investigative dermatology, vol. 139, no. 9, pp. 1889–1897.e4, 2019, ISSN: 1523-1747 (Electronic).

Abstract | Links | BibTeX

Reynier, Marie; Allart, Sophie; Goudounèche, Dominique; Moga, Alain; Serre, Guy; Simon, Michel; Leprince, Corinne

The Actin-Based Motor Myosin Vb Is Crucial to Maintain Epidermal Barrier Integrity. Journal Article

In: The Journal of investigative dermatology, vol. 139, no. 7, pp. 1430–1438, 2019, ISSN: 1523-1747 (Electronic).

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Albérola, Géraldine; Schröder, Jens-Michael; Froment, Carine; Simon, Michel

The Amino-Terminal Part of Human FLG2 Is a Component of Cornified Envelopes. Journal Article

In: The Journal of investigative dermatology, vol. 139, no. 6, pp. 1395–1397, 2019, ISSN: 1523-1747 (Electronic).

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Tauber, M; Apoil, P A; Richet, C; Laurent, J; De Bonnecaze, G; Mouchon, E; Cassagne, M; Marguery, M C; Hegazy, S; Konstantinou, M P; Severino, M; Uthurriague, C; Giordano-Labadie, F; Didier, A; Paul, C

Effect of dupilumab on atopic manifestations in patients treated for atopic dermatitis in real-life practice. Journal Article

In: vol. 180, no. 6, pp. 1551–1552, 2019, ISSN: 1365-2133 (Electronic).

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Pin, Didier; Pendaries, Valérie; Keita Alassane, Sokhna; Froment, Carine; Amalric, Nicolas; Cadiergues, Marie-Christine; Serre, Guy; Haftek, Marek; Vidémont, Emilie; Simon, Michel

Refined Immunochemical Characterization in Healthy Dog Skin of the Epidermal Cornification Proteins, Filaggrin, and Corneodesmosin. Journal Article

In: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, vol. 67, no. 2, pp. 85–97, 2019, ISSN: 1551-5044 (Electronic).

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Mazereeuw-Hautier, J; Vahlquist, A; Traupe, H; Bygum, A; Amaro, C; Aldwin, M; Audouze, A; Bodemer, C; Bourrat, E; Diociaiuti, A; Dolenc-Voljc, M; Dreyfus, I; El Hachem, M; Fischer, J; Gaanemo, A; Gouveia, C; Gruber, R; Hadj-Rabia, S; Hohl, D; Jonca, N; Ezzedine, K; Maier, D; Malhotra, R; Rodriguez, M; Ott, H; Paige, D G; Pietrzak, A; Poot, F; Schmuth, M; Sitek, J C; Steijlen, P; Wehr, G; Moreen, M; O'Toole, E A; Oji, V; Hernandez-Martin, A

Management of congenital ichthyoses: European guidelines of care, part one. Journal Article

In: The British journal of dermatology, vol. 180, no. 2, pp. 272–281, 2019, ISSN: 1365-2133 (Electronic).

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Malki, Liron; Sarig, Ofer; Romano, Maria-Teresa; Méchin, Marie-Claire; Peled, Alon; Pavlovsky, Mor; Warshauer, Emily; Samuelov, Liat; Uwakwe, Laura; Briskin, Valeria; Mohamad, Janan; Gat, Andrea; Isakov, Ofer; Rabinowitz, Tom; Shomron, Noam; Adir, Noam; Simon, Michel; McMichael, Amy; Dlova, Ncoza C; Betz, Regina C; Sprecher, Eli

Variant PADI3 in Central Centrifugal Cicatricial Alopecia. Journal Article

In: The New England journal of medicine, vol. 380, no. 9, pp. 833–841, 2019, ISSN: 1533-4406 (Electronic).

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Zingkou, Eleni; Pampalakis, Georgios; Kiritsi, Dimitra; Valari, Manthoula; Jonca, Nathalie; Sotiropoulou, Georgia

Activography reveals aberrant proteolysis in desquamating diseases of differing backgrounds. Journal Article

In: Experimental dermatology, vol. 28, no. 1, pp. 86–89, 2019, ISSN: 1600-0625 (Electronic).

Abstract | Links | BibTeX

2018

Cau, Laura; Méchin, Marie-Claire; Simon, Michel

Peptidylarginine deiminases and deiminated proteins at the epidermal barrier. Journal Article

In: Experimental dermatology, vol. 27, no. 8, pp. 852–858, 2018, ISSN: 1600-0625 (Electronic).

Abstract | Links | BibTeX

Hsu, Chiung-Yueh; Lecland, Nicolas; Pendaries, Valérie; Viodé, Cécile; Redoulès, Daniel; Paul, Carle; Merdes, Andreas; Simon, Michel; Bierkamp, Christiane

Stabilization of microtubules restores barrier function after cytokine-induced defects in reconstructed human epidermis. Journal Article

In: Journal of dermatological science, vol. 91, no. 1, pp. 87–96, 2018, ISSN: 1873-569X (Electronic).

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Zaafouri, Sarra; Pichery, Mélanie; Huchenq, Anne; Valentin, Frederic; Oji, Vinzenz; Mazereeuw-Hautier, Juliette; Serre, Guy; Jonca, Nathalie

Transcriptomic Analysis of Two Cdsn-Deficient Mice Shows Gene Signatures Biologically Relevant for Peeling Skin Disease. Journal Article

In: The journal of investigative dermatology, vol. 138, no. 6, pp. 1431–1435, 2018, ISSN: 1523-1747 (Electronic).

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Onnis, G; Bourrat, E; Jonca, N; Dreyfus, I; Severino-Freire, M; Pichery, M; Fischer, J; Mazereeuw-Hautier, J

KLICK syndrome: an unusual phenotype. Journal Article

In: British journal of dermatology, vol. 178, no. 6, pp. 1445–1446, 2018, ISSN: 1365-2133 (Electronic).

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2017

Cau, Laura; Pendaries, Valérie; Lhuillier, Emeline; Thompson, Paul R; Serre, Guy; Takahara, Hidenari; Méchin, Marie-Claire; Simon, Michel

Lowering relative humidity level increases epidermal protein deimination and drives human filaggrin breakdown. Journal Article

In: Journal of dermatological science, vol. 86, no. 2, pp. 106–113, 2017, ISSN: 1873-569X (Electronic).

Abstract | Links | BibTeX

Pichery, Mélanie; Huchenq, Anne; Sandhoff, Roger; Severino-Freire, Maella; Zaafouri, Sarra; Lukáš Opálka,; Levade, Thierry; Soldan, Vanessa; Bertrand-Michel, Justine; Lhuillier, Emeline; Serre, Guy; Maruani, Annabel; Mazereeuw-Hautier, Juliette; Jonca, Nathalie

PNPLA1 defects in patients with autosomal recessive congenital ichthyosis and KO mice sustain PNPLA1 irreplaceable function in epidermal omega-O-acylceramide synthesis and skin permeability barrier. Journal Article

In: Human molecular genetics, vol. 26, no. 10, pp. 1787–1800, 2017, ISSN: 1460-2083 (Electronic).

Abstract | Links | BibTeX

Hsu, Chiung-Yueh; Gasc, Géraldine; Raymond, Anne-Aurélie; Burlet-Schiltz, Odile; Takahara, Hidenari; Serre, Guy; Méchin, Marie-Claire; Simon, Michel

Deimination of Human Hornerin Enhances its Processing by Calpain-1 and its Cross-Linking by Transglutaminases. Journal Article

In: The Journal of investigative dermatology, vol. 137, no. 2, pp. 422–429, 2017, ISSN: 1523-1747 (Electronic).

Abstract | Links | BibTeX

2016

Ü Basmanav, F Buket; Cau, Laura; Tafazzoli, Aylar; Méchin, Marie-Claire; Wolf, Sabrina; Romano, Maria Teresa; Valentin, Frederic; Wiegmann, Henning; Huchenq, Anne; Kandil, Rima; Garcia Bartels, Natalie; Kilic, Arzu; George, Susannah; Ralser, Damian J; Bergner, Stefan; Ferguson, David J P; Oprisoreanu, Ana-Maria; Wehner, Maria; Thiele, Holger; Altmüller, Janine; Nürnberg, Peter; Swan, Daniel; Houniet, Darren; Büchner, Aline; Weibel, Lisa; Wagner, Nicola; Grimalt, Ramon; Bygum, Anette; Serre, Guy; Blume-Peytavi, Ulrike; Sprecher, Eli; Schoch, Susanne; Oji, Vinzenz; Hamm, Henning; Farrant, Paul; Simon, Michel; Betz, Regina C

Mutations in Three Genes Encoding Proteins Involved in Hair Shaft Formation Cause Uncombable Hair Syndrome. Journal Article

In: American journal of human genetics, vol. 99, no. 6, pp. 1292–1304, 2016, ISSN: 1537-6605 (Electronic).

Abstract | Links | BibTeX

Li, Jiagui; Leyva-Castillo, Juan Manuel; Hener, Pierre; Eisenmann, Aurelie; Zaafouri, Sarra; Jonca, Nathalie; Serre, Guy; Birling, Marie-Christine; Li, Mei

Counterregulation between thymic stromal lymphopoietin- and IL-23-driven immune axes shapes skin inflammation in mice with epidermal barrier defects. Journal Article

In: The Journal of allergy and clinical immunology, vol. 138, no. 1, pp. 150–161.e13, 2016, ISSN: 1097-6825 (Electronic).

Abstract | Links | BibTeX

Hotz, Alrun; Oji, Vinzenz; Bourrat, Emmanuelle; Jonca, Nathalie; Mazereeuw-Hautier, Juliette; Betz, Regina C; Blume-Peytavi, Ulrike; Stieler, Karola; Morice-Picard, Fanny; Schönbuchner, Ines; Markus, Susanne; Schlipf, Nina; Fischer, Judith

Expanding the Clinical and Genetic Spectrum of KRT1, KRT2 and KRT10 Mutations in Keratinopathic Ichthyosis. Journal Article

In: Acta dermato-venereologica, vol. 96, no. 4, pp. 473–478, 2016, ISSN: 1651-2057 (Electronic).

Abstract | Links | BibTeX

Maier, D; Mazereeuw-Hautier, J; Tilinca, M; Cosgarea, R; Jonca, N

Novel mutation in NIPAL4 in a Romanian family with autosomal recessive congenital ichthyosis. Journal Article

In: Clinical and experimental dermatology, vol. 41, no. 3, pp. 279–282, 2016, ISSN: 1365-2230 (Electronic).

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Tauber, Marie; Balica, Stefana; Hsu, Chiung-Yueh; Jean-Decoster, Catherine; Lauze, Christophe; Redoules, Daniel; Viodé, Cécile; Schmitt, Anne-Marie; Serre, Guy; Simon, Michel; Paul, Carle F

Staphylococcus aureus density on lesional and nonlesional skin is strongly associated with disease severity in atopic dermatitis. Journal Article

In: The journal of allergy and clinical immunology, vol. 137, no. 4, pp. 1272–1274.e3, 2016, ISSN: 1097-6825 (Electronic).

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Malaisse, Jérémy; Pendaries, Valérie; Hontoir, Fanny; De Glas, Valérie; Van Vlaender, Daniel; Simon, Michel; Lambert de Rouvroit, Catherine; Poumay, Yves; Flamion, Bruno

Hyaluronan Does Not Regulate Human Epidermal Keratinocyte Proliferation and Differentiation. Journal Article

In: The Journal of biological chemistry, vol. 291, no. 12, pp. 6347–6358, 2016, ISSN: 1083-351X (Electronic).

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Méchin, Marie-Claire; Cau, Laura; Galliano, Marie-Florence; Daunes-Marion, Sylvie; Poigny, Stéphane; Vidaluc, Jean-Louis; Bessou-Touya, Sandrine; Takahara, Hidenari; Serre, Guy; Duplan, Hélène; Simon, Michel

Acefylline activates filaggrin deimination by peptidylarginine deiminases in the upper epidermis. Journal Article

In: Journal of dermatological science, vol. 81, no. 2, pp. 101–106, 2016, ISSN: 1873-569X (Electronic).

Abstract | Links | BibTeX

2015

Valentin, Frederic; Oji, Vinzenz; Hausser, Ingrid; Liebau, Eva; Tarinski, Tatjana; Metze, Dieter; Breitkreutz, Dirk; Traupe, Heiko; Jonca, Nathalie; Terheyden, Patrick

Increased expression of caspase-1 and interleukin-18 in peeling skin disease, and a novel mutation of corneodesmosin. Journal Article

In: Acta dermato-venereologica, vol. 95, no. 8, pp. 1019–1021, 2015, ISSN: 1651-2057 (Electronic).

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Le Lamer, Marina; Pellerin, Laurence; Reynier, Marie; Cau, Laura; Pendaries, Valérie; Leprince, Corinne; Méchin, Marie-Claire; Serre, Guy; Paul, Carle; Simon, Michel

Defects of corneocyte structural proteins and epidermal barrier in atopic dermatitis. Journal Article

In: Biological chemistry, vol. 396, no. 11, pp. 1163–1179, 2015, ISSN: 1437-4315 (Electronic).

Abstract | Links | BibTeX

Pendaries, V; Le Lamer, M; Cau, L; Hansmann, B; Malaisse, J; Kezic, S; Serre, G; Simon, M

In a three-dimensional reconstructed human epidermis filaggrin-2 is essential for proper cornification. Journal Article

In: Cell death & disease, vol. 6, no. 2, pp. e1656, 2015, ISSN: 2041-4889 (Electronic).

Abstract | Links | BibTeX

2014

Pendaries, Valérie; Malaisse, Jeremy; Pellerin, Laurence; Le Lamer, Marina; Nachat, Rachida; Kezic, Sanja; Schmitt, Anne-Marie; Paul, Carle; Poumay, Yves; Serre, Guy; Simon, Michel

Knockdown of filaggrin in a three-dimensional reconstructed human epidermis impairs keratinocyte differentiation. Journal Article

In: The Journal of investigative dermatology, vol. 134, no. 12, pp. 2938–2946, 2014, ISSN: 1523-1747 (Electronic).

Abstract | Links | BibTeX

Pellerin, Laurence; Paul, Carle; Schmitt, Anne-Marie; Serre, Guy; Simon, Michel

Bleomycin hydrolase downregulation in lesional skin of adult atopic dermatitis patients is independent of FLG gene mutations. Journal Article

In: The Journal of allergy and clinical immunology, vol. 134, no. 6, pp. 1459–1461.e7, 2014, ISSN: 1097-6825 (Electronic).

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Bergboer, Judith G M; Dulak, Maria G; van Vlijmen-Willems, Ivonne M J J; Jonca, Nathalie; van Wijk, Erwin; Hendriks, Wiljan J A J; Zeeuwen, Patrick L J M; Schalkwijk, Joost

Analysis of protein-protein interaction between late cornified envelope proteins and corneodesmosin. Journal Article

In: The journal of investigative dermatology, vol. 23, no. 10, pp. 769–771, 2014, ISSN: 1600-0625 (Electronic).

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Fredonnet, Julie; Gasc, Géraldine; Serre, Guy; Séverac, Childérick; Simon, Michel

Topographical and nano-mechanical characterization of native corneocytes using atomic force microscopy. Journal Article

In: Journal of dermatological science, vol. 75, no. 1, pp. 63–65, 2014, ISSN: 1873-569X (Electronic).

Links | BibTeX

Leclerc, Emilie A; Huchenq, Anne; Kezic, Sanja; Serre, Guy; Jonca, Nathalie

Mice deficient for the epidermal dermokine $beta$ and $gamma$ isoforms display transient cornification defects. Journal Article

In: Journal of cell science, vol. 127, no. Pt 13, pp. 2862–2872, 2014, ISSN: 1477-9137 (Electronic).

Abstract | Links | BibTeX

Hayez, Aurélie; Malaisse, Jérémy; Roegiers, Edith; Reynier, Marie; Renard, Chantal; Haftek, Marek; Geenen, Vincent; Serre, Guy; Simon, Michel; de Rouvroit, Catherine Lambert; Michiels, Carine; Poumay, Yves

High TMEM45A expression is correlated to epidermal keratinization. Journal Article

In: Experimental dermatology, vol. 23, no. 5, pp. 339–344, 2014, ISSN: 1600-0625 (Electronic).

Abstract | Links | BibTeX

Kezic, Sanja; Novak, Natalija; Jakasa, Ivone; Jungersted, Jackob M; Simon, Michel; Brandner, Johanna M; Middelkamp-Hup, Maritza A; Weidinger, Stephan

Skin barrier in atopic dermatitis. Journal Article

In: Frontiers in bioscience (Landmark edition), vol. 19, pp. 542–556, 2014, ISSN: 1093-4715 (Electronic).

Abstract | Links | BibTeX

2013

Mallet, A; Kypriotou, M; George, K; Leclerc, E; Rivero, D; Mazereeuw-Hautier, J; Serre, G; Huber, M; Jonca, N; Hohl, D

Identification of the first nonsense CDSN mutation with expression of a truncated protein causing peeling skin syndrome type B. Journal Article

In: The British journal of dermatology, vol. 169, no. 6, pp. 1322–1325, 2013, ISSN: 1365-2133 (Electronic).

Abstract | Links | BibTeX

Pellerin, Laurence; Henry, Julie; Hsu, Chiung-Yueh; Balica, Stéfana; Jean-Decoster, Catherine; Méchin, Marie-Claire; Hansmann, Britta; Rodriguez, Elke; Weindinger, Stefan; Schmitt, Anne-Marie; Serre, Guy; Paul, Carle; Simon, Michel

Defects of filaggrin-like proteins in both lesional and nonlesional atopic skin. Journal Article

In: The Journal of allergy and clinical immunology, vol. 131, no. 4, pp. 1094–1102, 2013, ISSN: 1097-6825 (Electronic).

Abstract | Links | BibTeX

2012

de Koning, H D; van den Bogaard, E H; Bergboer, J G M; Kamsteeg, M; van Vlijmen-Willems, I M J J; Hitomi, K; Henry, J; Simon, M; Takashita, N; Ishida-Yamamoto, A; Schalkwijk, J; Zeeuwen, P L J M

Expression profile of cornified envelope structural proteins and keratinocyte differentiation-regulating proteins during skin barrier repair. Journal Article

In: The British journal of dermatology, vol. 166, no. 6, pp. 1245–1254, 2012, ISSN: 1365-2133 (Electronic).

Abstract | Links | BibTeX

Lagarrigue, Sophie Garrido; George, Jerome; Questel, Emmanuel; Lauze, Christophe; Meyer, Nicolas; Lagarde, Jean-Michel; Simon, Michel; Schmitt, Anne-Marie; Serre, Guy; Paul, Carle

In vivo quantification of epidermis pigmentation and dermis papilla density with reflectance confocal microscopy: variations with age and skin phototype. Journal Article

In: Experimental dermatology, vol. 21, no. 4, pp. 281–286, 2012, ISSN: 1600-0625 (Electronic).

Abstract | Links | BibTeX

Mazereeuw-Hautier, J; Dreyfus, I; Barbarot, S; Serrentino, L; Bourdon-Lanoy, E; Ezzedine, K; Maza, A; Aujoulat, I; Le Rhun, A

Factors influencing quality of life in patients with inherited ichthyosis: a qualitative study in adults using focus groups. Journal Article

In: The British journal of dermatology, vol. 166, no. 3, pp. 646–648, 2012, ISSN: 1365-2133 (Electronic).

Abstract | Links | BibTeX

Henry, Julie; Toulza, Eve; Hsu, Chiung-Yueh; Pellerin, Laurence; Balica, Stefana; Mazereeuw-Hautier, Juliette; Paul, Carle; Serre, Guy; Jonca, Nathalie; Simon, Michel

Update on the epidermal differentiation complex. Journal Article

In: Frontiers in bioscience (Landmark edition), vol. 17, pp. 1517–1532, 2012, ISSN: 1093-4715 (Electronic).

Abstract | Links | BibTeX

2011

Mazereeuw-Hautier, J; Leclerc, E A; Simon, M; Serre, G; Jonca, N

A novel mutation in CDSN causes peeling skin disease in a patient from Morocco. Journal Article

In: British journal of dermatology, vol. 165, no. 5, pp. 1152–1155, 2011, ISSN: 1365-2133 (Electronic).

Links | BibTeX

Bodemer, C; Bourrat, E; Mazereeuw-Hautier, J; Boralevi, F; Barbarot, S; Bessis, D; Blanchet-Bardon, C; Bourdon-Lanoy, E; Stalder, J-F; Ribet, V; Guerrero, D; Sibaud, V

Short- and medium-term efficacy of specific hydrotherapy in inherited ichthyosis. Journal Article

In: The British journal of dermatology, vol. 165, no. 5, pp. 1087–1094, 2011, ISSN: 1365-2133 (Electronic).

Abstract | Links | BibTeX

Hsu, Chiung-Yueh; Henry, Julie; Raymond, Anne-Aurélie; Méchin, Marie-Claire; Pendaries, Valérie; Nassar, Dany; Hansmann, Britta; Balica, Stéfana; Burlet-Schiltz, Odile; Schmitt, Anne-Marie; Takahara, Hidenari; Paul, Carle; Serre, Guy; Simon, Michel

Deimination of human filaggrin-2 promotes its proteolysis by calpain 1. Journal Article

In: The Journal of biological chemistry, vol. 286, no. 26, pp. 23222–23233, 2011, ISSN: 1083-351X (Electronic).

Abstract | Links | BibTeX

Jonca, Nathalie; Caubet, Cécile; Leclerc, Emilie A; Guerrin, Marina; Simon, Michel; Serre, Guy

Protease sensitivity of corneodesmosin variants encoded by the six more common CDSN haplotypes. Journal Article

In: The journal of investigative dermatology, vol. 131, no. 6, pp. 1381–1384, 2011, ISSN: 1523-1747 (Electronic).

Links | BibTeX

Henry, Julie; Hsu, Chiung-Yueh; Haftek, Marek; Nachat, Rachida; de Koning, Heleen D; Gardinal-Galera, Isabelle; Hitomi, Kiyotaka; Balica, Stéfana; Jean-Decoster, Catherine; Schmitt, Anne-Marie; Paul, Carle; Serre, Guy; Simon, Michel

Hornerin is a component of the epidermal cornified cell envelopes. Journal Article

In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, vol. 25, no. 5, pp. 1567–1576, 2011, ISSN: 1530-6860 (Electronic).

Abstract | Links | BibTeX

Jonca, Nathalie; Leclerc, Emilie A; Caubet, Cécile; Simon, Michel; Guerrin, Marina; Serre, Guy

Corneodesmosomes and corneodesmosin: from the stratum corneum cohesion to the pathophysiology of genodermatoses. Journal Article

In: European journal of dermatology : EJD, vol. 21 Suppl 2, pp. 35–42, 2011, ISSN: 1167-1122 (Print).

Abstract | Links | BibTeX

Leclerc, Emilie A; Gazeilles, Leila; Serre, Guy; Guerrin, Marina; Jonca, Nathalie

The ubiquitous dermokine delta activates Rab5 function in the early endocytic pathway. Journal Article

In: PloS one, vol. 6, no. 3, pp. e17816, 2011, ISSN: 1932-6203 (Electronic).

Abstract | Links | BibTeX

Mattiuzzo, Nicolas R; Toulza, Eve; Jonca, Nathalie; Serre, Guy; Guerrin, Marina

A large-scale multi-technique approach identifies forty-nine new players of keratinocyte terminal differentiation in human epidermis. Journal Article

In: Experimental dermatology, vol. 20, no. 2, pp. 113–118, 2011, ISSN: 1600-0625 (Electronic).

Abstract | Links | BibTeX

Vasilopoulos, Yiannis; Sharaf, Nazar; di Giovine, Franco; Simon, Michel; Cork, Michael J; Duff, Gordon W; Tazi-Ahnini, Rachid

The 3'-UTR AACCins5874 in the stratum corneum chymotryptic enzyme gene (SCCE/KLK7), associated with atopic dermatitis; causes an increased mRNA expression without altering its stability. Journal Article

In: Journal of dermatological science, vol. 61, no. 2, pp. 131–133, 2011, ISSN: 1873-569X (Electronic).

Links | BibTeX

Coudane, Fanny; Méchin, Marie-Claire; Huchenq, Anne; Henry, Julie; Nachat, Rachida; Ishigami, Akihito; Adoue, Véronique; Sebbag, Mireille; Serre, Guy; Simon, Michel

Deimination and expression of peptidylarginine deiminases during cutaneous wound healing in mice. Journal Article

In: European journal of dermatology : EJD, vol. 21, no. 3, pp. 376–384, 2011, ISSN: 1167-1122 (Print).

Abstract | Links | BibTeX

Paul, C; Maumus-Robert, S; Mazereeuw-Hautier, J; Guyen, C N; Saudez, X; Schmitt, A M

Prevalence and risk factors for xerosis in the elderly: a cross-sectional epidemiological study in primary care. Journal Article

In: Dermatology (Basel, Switzerland), vol. 223, no. 3, pp. 260–265, 2011, ISSN: 1421-9832 (Electronic).

Abstract | Links | BibTeX

Collaborations

National collaborations:

Dr N. GAUDENZIO, Institut Infinity, Toulouse

Dr N. FAZILLEAU, Institut Infinity, Toulouse

Pr R. POUPOT, Institut Infinity, Toulouse

Pr B. VELAS, Gérontopole, CHU de Toulouse

Pr N. MERBAHI, Laboratoire LAPLACE, Toulouse

Dr V. PLANAT, Laboratoire RESTORE, Toulouse

Pr T. LEVADE, Centre de Recherches en Cancérologie de Toulouse

Dr C.-O. TURRIN, Laboratoire de Chimie de Coordination, Toulouse

Dr M. BLANZAT, Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique, Toulouse

Dr S. BESSOU-TOUYA et Dr H. DUPLAN, Pierre Fabre Dermo-Cosmétique, Toulouse

Dr P. DESCARGUES, Genoskin, Toulouse

Dr M. HAFTEK, Université Lyon 1, Lyon

Dr D. PIN, Ecole Nationale Vétérinaire de Lyon

Dr S. PAIN, BASF Beauty Care Solutions France, Lyon

Pr B. VABRES, Service d’Ophtalmologie, CHU de Nantes

Dr D. BERNARD, L’Oréal, Paris


International collaborations:

Pr Y. POUMAY, Laboratoire de physiologie moléculaire (URPHYM), Université de Namur, Belgique

Dr S. KEZIC, University of Amsterdam, Coronel Institute of Occupational Health, Amsterdam, Netherlands

Dr J. LYSELL, Dermatology and Venereology division, Karolinska Institutet in Stockholm, Sweden

Pr R. BETZ, Institute of Human Genetics, University of Bonn, Germany

Pr R. SANDHOFF, Department of Cellular and Molecular Pathology, German CanCer Research Centre (DKFZ), Heidelberg, Germany

Pr G. SOTIROPOULOU, Department of Pharmacy, School of Health Sciences, University of Patras, Greece

Pr L. OPALKA, Department of Inorganic and Organic Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University in Prague, Czech Republic

Pr E. SPRECHER, Department of Dermatology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel

Pr P. THOMPSON Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts, USA

European Epidermal Barrier Research Network (E2BRN ; www.e2brn.net)

 

 
Impact on the society

Our team aims to decipher the molecular determinants of this physical, biochemical and immunological barrier to the external environment that is the epidermis. At the same time, we try to understand how alterations in the epidermal barrier are responsible for several skin diseases, some very rare such as ichthyoses, others very common such as eczema. The ultimate goal is to discover new treatment options. To do this, we develop innovative experimental models and collaborate with clinical services and two Rare Disease Reference Centre at the Toulouse University Hospital.

Our team is made up of statutory and young CDD staff from a wide range of backgrounds (medical, scientific, veterinary, academic, school of engineering, etc.). It is based on complementary expertise in immunology, cellular and molecular biology, biochemistry and genetics. The resulting scientific environment allows fundamental and translational research to be developed.

We enjoy a strong international visibility in the field of dermatology. We were able to build a vast network of national, European and non-European academic collaborations, and multiple partnerships with major industrial groups (Pierre Fabre Dermo-Cosmétique, L’Oréal and BASF) and local private companies (Silab, Synelvia, Genoskin, etc.). Several patents are also operated under license with royalties that contribute to the financing of our research. These interactions with the industrial world and our projects with high valuation potential are strongly supported by Inserm Transfert, Toulouse Tech Transfer and the Occitanie/ Pyrénées – Méditerranée Region.

Many of us actively participate in the teachings of the Faculty of Medicine in Toulouse, and we regularly form to the research careers young scientists, PhD students and trainees of all levels.

Alumni

PhD Students:
  • Véronique Adoue
  • Laura Cau
  • Fanny Coudane
  • Sophie Garrido-Lagarrigue
  • Julie Henry
  • Marina Le Lamer
  • Émilie Leclerc
  • Tatiana Lupasco
  • Alicia Mallet
  • Nicolas Mattuzzio
  • Laurence Pellerin
  • Marie Reynier
  • Eve Toulza
  • Sara Zaafouri

Post-Doctoral Fellow:
  • Cécile Caubet
  • Chiung-Yueh Hsu
  • Sohna Keita Alassane
  • Rachida Nachat
  • Valérie Pendaries
  • Mélanie Pichery

INSERM

INSERM

CNRS

CNRS

Université Toulouse Paul Sabatier

Université Toulouse Paul Sabatier

Région Occitanie

Région Occitanie

Université Fédérale Toulouse Midi-Pyrénées

Université Fédérale Toulouse Midi-Pyrénées

Agence Nationale de la Recherche

Agence Nationale de la Recherche

Société Française de Dermatologie (SFD)

Société Française de Dermatologie (SFD)

École Nationale Vétérinaire de Toulouse

École Nationale Vétérinaire de Toulouse

Centre de référence des maladies rares de la peau

Centre de référence des maladies rares de la peau

CHU

CHU

PHENOMIN

PHENOMIN

TTT

TTT

Société de Recherche Dermatologique (SRD)

Société de Recherche Dermatologique (SRD)

Société Française d’Allergologie (SFA)

Société Française d’Allergologie (SFA)

Association Ichtyose France (AIF)

Association Ichtyose France (AIF)

European Barrier Epidermal Research Network (E2BRN)

European Barrier Epidermal Research Network (E2BRN)

Centre de référence du kératocône

Centre de référence du kératocône

ERARE

ERARE

INSPIRE

INSPIRE