Viral infection : persistence, host response and pathophysiology

Coordinators : J. Izopet, B. Lagane

Scientific Objectives


Our team specializes in the pathophysiology of infections with human immunodeficiency virus (HIV), hepatitis E virus (HEV) and zika virus (ZIKV). The major specific topic is the mechanisms underlying the persistence of virus in immunocompetent hosts (HIV, ZIKV) and immunocompromised patients (HEV). We are also working on how HIV enters host cells via chemokine receptors and the pathophysiology of HIV infection in the intestinal mucosa. Another aspect of our work is the route of transmission and pathogenesis of HEV and ZIKV during pregnancy. We have developed a number of specific model cell systems, including placental explants, extravillous cytotrophoblasts, hepatocytes, enterocytes, peripheral and tissue lymphocytes, together with systems based on clinical samples (intestinal tissue, early and term placenta, blood and biopsies from immunocompromised patients).

Our projects


AXIS 1: HIV

Our laboratory develops a wide array of approaches in the fields of immunology, virology, molecular pharmacology and structural biology to study important aspects of the pathophysiology of HIV infection and AIDS. We are deeply anchored at the interface between basic and clinical science and benefit from the proximity of the Toulouse University Hospital. Our research comprises two main axes, which are themselves highly interconnected:


Mechanisms of HIV-1 entry and their pathophysiological consequences

PI: B. Lagane; Participants: P. Colin, B. Puissant, S. Raymond, M. Armani

We study the properties of interactions between the HIV-1 envelope glycoproteins (Env) and their cellular receptors, i.e. CD4 and depending on the viral tropism, the coreceptors CCR5 or CXCR4. We investigate how these interactions are regulated by factors of the target cells and the host immune responses and conversely, how the virus alters the cellular functions and immunity of the host. Finally we seek to characterize how the properties of viral entry shape the phenotypic properties of viruses and their role in different processes and stages of HIV-1 infection. These include first the very earliest step of infection, that is transmission of the virus. We seek to identify whether Env determinants lead to virus selection during transmission and favor the first steps in the establishment of infection in the host (WP #1). We also study how the properties of viral entry change over time in infection and by so doing contribute to pathogenesis of infection, in particular depletion of CD4 T-cells. In this regard, we investigate the role that the coreceptor natural ligands, i.e. several CC chemokines for CCR5 and CXCL12 for CXCR4, could play as a driving force of evolution of the properties of CCR5- and CXCR4-using viruses (WP #2). A third project is dealing with how the properties of Env interactions with cellular receptors regulate the cellular tropism of viruses, in particular their capacity to enter into macrophages. These cells play a critical role in the pathophysiology of HIV-1 infection. They form viral reservoirs and contribute to dissemination of the virus in tissues, in particular the central nervous systems, which has been linked to development of neurological disorders in patients. On this topic, we are currently pursuing our recent work (Colin, PLoS Pathog., 2018) on the role of CCR5 conformational heterogeneity in HIV-1 entry into macrophages, both in the context of infection by cell free viruses and through CD4 T-cells/macrophages contacts (WP #3). Lastly, our research deals with the signaling properties of primary Env/CCR5 interactions, which are thought to contribute to many aspects of the pathophysiology of infection. We speculate that signaling of Envs might depend on the CCR5 conformation(s) to which they bind, and that this process contributes to differences in the phenotypic properties between distinct HIV-1 strains. We are currently investigating this using BRET-based methods to monitor in real-time and in living cells the most upstream events of CCR5 signaling, i.e. interactions with G proteins and arrestins (WP #4).

HIV-1 persistence and impaired immune reconstitution in the gut mucosa despite antiretroviral therapy

PI: Prof. P. Delobel; Participants: M. Requena, C. Vellas, N. Collercandy

The gut mucosa remains substantially depleted of CD4+ T cells in HIV-1-infected individuals despite antiretroviral therapy, contrasting with much more complete restoration of CD4+ T cell counts in the blood and organized lymphoid tissues. The reasons for this dissociation remain largely unknown. The impaired reconstitution of the immune gut barrier is clinically relevant as it is associated with persistent microbial translocation events and chronic immune activation that lead to inflammation-related premature ageing in treated HIV-1-infected individuals.

We contributed to a better understanding of the mechanisms involved in the impaired immune reconstitution of the gut mucosa by describing alterations in the recruitment of CD4+ T cells, notably of Th17 cells, a cell subset playing a major role in mucosal defense against bacteria and fungi.

We found that small intestine epithelial cells produce reduced amounts of the CCL20 and CCL25 chemokines in HIV-1-infected individuals on antiretroviral therapy, leading to a defective recruitment of CCR6+ and CCR9+ immune cells, notably Th17 cells (Mavigner et al., J Clin Invest, 2012; Loiseau et al., Mucosal Immunol, 2016). By contrast, Th1 and effector memory CD8+ T cells could efficiently be recruited via alternative chemotactic axes, notably through CXCR3-mediated chemotactism (Loiseau et al., J Infect Dis, 2019). Further, Th1 and effector memory CD8+ T cells contribute to altering the recruitment of Th17 cells by IFN-g- and IL-18-mediated reduction of CCL20 production by enterocytes. By contrast, Treg cells remain efficiently recruited to the gut mucosa, leading to a sustained imbalance between Th17 and Treg cells, which is a hallmark of HIV-1 infection. We also found that Th22 cells could use the CCL28-CCR10 axis as an alternative to CCL20-CCR6 to migrate to the gut mucosa in treated HIV-1-infected individuals. But the restoration of Th22 cells is not sufficient in reducing persistent microbial translocation (Nayrac et al., Mucosal Immunol, 2020).

We also developed ex-vivo models to reproduce the gut mucosal microenvironment. We notably succeed in culturing primary human enterocytes, and driving their differentiation to reconstitute a polarized mature gut epithelium on transwells, allowing co-cultures with T cells. We also developed a model of gut tissue histocultures that could be infected ex vivo by HIV-1 to analyze the virus target cells in the gut microenvironment.

Another axis of our research focuses on HIV-1 persistence in the gut mucosa despite effective antiretroviral therapy. We developed several molecular tools allowing in-depth characterization of the HIV-1 reservoir in the blood and gut compartments, notably qPCR of residual RNA and DNA HIV-1 (tissue and cell-associated RNA, total and integrated DNA), ddPCR quantification of intact proviruses, single copy NGS of integrated proviruses (Illumina), analysis of the virus quasispecies genetic complexity by NGS (PacBio).

HIV-1 persistence in the gut leads to local and systemic chronic inflammation through sustained antigenic stimulation and immune effector cells recruitment to the mucosa. We are studying the role of IDO-1 in inducing Th17/Treg imbalance and T cell exhaustion, and the relationships between the gut microbiota and HIV-1 persistence in the gut mucosa.

 

Our research mainly relies on gut endoscopic samples obtain from cohorts of HIV-1-infected individuals and uninfected controls, recruited at Toulouse University Hospital in the ANRS EP44 and EP61 GALT studies (PI, Pr P. Delobel). Our research also relies on BSL3 facilities, flow-cytometric analysis and cell sorting, NGS, and bioinformatic cores.

AXIS 2: HEV

Pathogenesis of HEV infection

Role of gamma-delta T cells during HEV infections

PI : E. Champagne ; Participants: F. Abravanel, H. El Costa.

Hepatitis E virus (HEV, genotype-3) causes chronic infections in immunosuppressed patients. Viral persistence is linked to the immunological status of the patients but the determinants are largely unknown. We are committed to investigate γδ T cells in the context of human viral infections and we have observed that γδ T cells present activation and mobilization markers in immunosuppressed patients at the acute phase of HEV infection.  We aim to clarify their role and if their activation is beneficial or detrimental. Our work is based on the analysis of patient blood samples collected at the acute, resolving or chronic phase of infection. Although rarely symptomatic, acute infections in immunocompetent individuals are sometimes diagnosed and allow investigating antiviral immunity in an immunocompetent context. γδ T cell reactivity against HEV-infected cells is also studied in in vitro models of infection and this has revealed that the virus is able to modulate the cytokine responses of γδ T cells to promote regulatory properties. Our objective is to determine if the immunomodulatory properties of HEV operate in vivo and can be targeted for therapeutic purposes.

Pathogenesis of Hepatitis E virus in high-risk patients: Role of the immune response.

PI : H. El Costa ; Participants : Q. Glaziou, N. Jabrane-Ferrat, F. Abravanel, J. Izopet.

HEV is currently the leading cause of acute viral hepatitis worldwide. The occurrence of severe clinical symptoms upon HEV infection increases during pregnancy, with aging and in immunosuppressed patients. Host immune response, rather than the virus itself, seems to be involved in the burden of HEV. Within this context, we recently showed that Effector memory CD8 T cell response elicits HEV pathogenesis in the elderly. Indeed, symptomatic infections were characterized by an expansion of highly activated effector memory CD8 T cells, regardless of antigen specificity. This robust activation was associated with qualitative and quantitative alterations in cytokine production. In addition, effector memory cells were endowed with high cytotoxic capacity and ability to rapidly migrate to the liver. Immune cell functions are tightly controlled by their energetic metabolism (including glucose, glutamine and fatty acids the pathways) which can be altered with age and following viral infections. Moreover, any alteration in one of the metabolic pathways leads not only to inefficient immune responses, but also to averse clinical outcomes. Thus, our ongoing studies aim at characterizing the response and the metabolic reprogramming of immune cells during HEV infection in the 3 high-risk groups of patients (pregnant women, elderly subjects and patients undergoing immunosuppressive treatment). Our goal is to determine the mechanisms responsible for viral clearance or occurrence of clinical complications.

HEV life cycle in hepatocytes and enterocytes

Role of autophagy in HEV budding from polarized hepatocytes

PI: S. Chapuy-Regaud ; Participants: J. Izopet, C. Allioux.

The budding of the quasi-enveloped form of the Hepatitis E Virus (eHEV) has been shown to depend on the exocytosis pathway. We developed a system of HEV culture on polarized cells which allows reproducing main features of hepatocytes (including polarized albumin and bile acid secretion and ZO-1 expression). In this system, eHEV is released in an infectious form mainly to the bile side; our preliminary results also show the role of autophagy. Our aim is to understand how exocytosis and autophagy are hijacked by eHEV for its egress and interplay at the molecular level. To this end, we study the characteristics of eHEV particles by proteomics and lipidomics and the intracellular events by shRNA knockdown of target proteins and imaging.

Replication of HEV in enterocytes

PI: J. Izopet ; Participants: O. Marion, M. Migueres, S. Lhomme.

The intestinal epithelium is the first barrier that HEV must cross before it can enter the portal bloodstream and gain access to the liver. The mechanisms by which HEV penetrates the gut epithelium tight-junction barrier are unknown. Animal model data indicate that the gut is an important site of extra-hepatic HEV replication and the human adenocarcinoma Caco-2 cell line is permissive to HEV. Our data from patients for whom ribavirin failed suggest that the gut could be an important virus reservoir. The role of intestinal M cells in HEV replication or transcytosis is unknown. Our preliminary data from cultures of primary small intestine epithelial cells polarized on transwell inserts indicate that enterocytes are permissive to HEV-1 and HEV-3 and release eHEV particles, preferentially from the apical side. Our main objectives are: (1) to study the localization of HEV in the intestinal mucosa after ex vivo infection of intestinal explants (2) to study changes in the permeability of epithelial cells induced by HEV infection (3) to determine the subcellular location of virus proteins (4) to evaluate the role of M cells in HEV replication or transcytosis (5) to study the effect of ribavirin and alpha interferon on virus production (6) to study innate immune signaling pathways following virus entry (HEV-1 vs HEV-3) and cytokine production.

AXIS 3: ZIKV

We are studying the replication of ZIKV in the genital compartment and at the maternofetal interface. We have evidence that ZIKV replicates differentially in a wide range of maternal and fetal cells, including decidual fibroblasts and macrophages, trophoblasts and umbilical cord mesenchymal stem cells.

Our latest results show that the virus persists in the semen of infected patients for a long time and that the spermatozoa bear viral antigens. We are now looking at the mechanisms the virus uses to persist in the genital compartment.

Other details


Publications

2020

Chen, Qian ; Gouilly, Jordi ; Ferrat, Yann J; Espino, Ana ; Glaziou, Quentin ; Cartron, Géraldine ; El Costa, Hicham ; Al-Daccak, Reem ; Jabrane-Ferrat, Nabila

Metabolic reprogramming by Zika virus provokes inflammation in human placenta Journal Article

Nature Communications, 11 (1), 2020, ISSN: 2041-1723.

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Nayrac, Manon ; Requena, Mary ; Loiseau, Claire ; Cazabat, Michelle ; Suc, Bertrand ; Carrere, Nicolas ; Barange, Karl ; Alric, Laurent ; Martin-Blondel, Guillaume ; Izopet, Jacques ; Delobel, Pierre

Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals Journal Article

Mucosal Immunology, 14 (1), pp. 219–228, 2020, ISSN: 1933-0219, 1935-3456.

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Raymond, Stéphanie ; Piffaut, Marie ; Bigot, Jonathan ; Cazabat, Michelle ; Montes, Brigitte ; Bertrand, Kevin ; Martin-Blondel, Guillaume ; Izopet, Jacques ; Delobel, Pierre

Sexual transmission of an extensively drug-resistant HIV-1 strain Journal Article

The Lancet HIV, 7 (8), pp. e529–e530, 2020, ISSN: 23523018.

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Marion, Olivier ; Lhomme, Sebastien ; Nayrac, Manon ; Dubois, Martine ; Pucelle, Mélanie ; Requena, Mary ; Migueres, Marion ; Abravanel, Florence ; Peron, Jean Marie ; Carrere, Nicolas ; Suc, Bertrand ; Delobel, Pierre ; Kamar, Nassim ; Izopet, Jacques

Hepatitis E virus replication in human intestinal cells Journal Article

Gut, 69 (5), pp. 901–910, 2020, ISSN: 0017-5749, 1468-3288.

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2019

Raymond, Stéphanie ; Jeanne, Nicolas ; Nicot, Florence ; Lefebvre, Caroline ; Carcenac, Romain ; Minier, Luce ; Chiabrando, Julie ; Cazabat, Michelle ; Delobel, Pierre ; Izopet, Jacques

Long-term evolution of transmitted CXCR4-using HIV-1 under effective antiretroviral therapy Journal Article

AIDS (London, England), 33 (13), pp. 1977–1985, 2019, ISSN: 1473-5571.

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Dimeglio, Chloé ; Raymond, Stéphanie ; Jeanne, Nicolas ; Reynes, Christelle ; Carcenac, Romain ; Lefebvre, Caroline ; Cazabat, Michelle ; Nicot, Florence ; Delobel, Pierre ; Izopet, Jacques

THETA: A new genotypic approach for predicting HIV-1 CRF02-AG coreceptor usage Journal Article

Bioinformatics (Oxford, England), 2019, ISSN: 1367-4811.

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Marion, O; Lhomme, S; Del Bello, A; Abravanel, F; Esposito, L; Hebral, A L; Lavayssiere, L; Cointault, O; Ribes, D; Izopet, J; Kamar, N

Monitoring hepatitis E virus fecal shedding to optimize ribavirin treatment duration in chronically infected transplant patients Journal Article

J Hepatol, 70 (1), pp. 206-209, 2019, ISSN: 1600-0641 (Electronic) 0168-8278 (Linking).

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Assoumou, L; Bocket, L; Pallier, C; Grude, M; Ait-Namane, R; Izopet, J; Raymond, S; Charpentier, C; Visseaux, B; Wirden, M; Trabaud, M A; Le Guillou-Guillemette, H; Allaoui, C; Henquell, C; Krivine, A; Dos Santos, G; Delamare, C; Bouvier-Alias, M; Montes, B; Ferre, V; De Monte, A; Signori-Schmuck, A; Maillard, A; Morand-Joubert, L; Tumiotto, C; Fafi-Kremer, S; Amiel, C; Barin, F; Marque-Juillet, S; Courdavault, L; Vallet, S; Beby-Defaux, A; de Rougemont, A; Fenaux, H; Avettand-Fenoel, V; Allardet-Servent, A; Plantier, J C; Peytavin, G; Calvez, V; Chaix, M L; Descamps, D; Group, Anrs Ac- Resistance Study

Stable prevalence of transmitted drug resistance mutations and increased circulation of non-B subtypes in antiretroviral-naive chronically HIV-infected patients in 2015/2016 in France Journal Article

J Antimicrob Chemother, 2019, ISSN: 1460-2091 (Electronic) 0305-7453 (Linking).

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Dimeglio, C; Kania, D; Mantono, J M; Kagone, T; Zida, S; Tassembedo, S; Dicko, A; Tinto, B; Yaro, S; Hien, H; Rouamba, J; Bicaba, B; Medah, I; Meda, N; Traore, O; Tuaillon, E; Abravanel, F; Izopet, J

Hepatitis E Virus Infections among Patients with Acute Febrile Jaundice in Burkina Faso Journal Article

Viruses, 11 (6), 2019, ISSN: 1999-4915 (Electronic) 1999-4915 (Linking).

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Dimeglio, C; Raymond, S; Nicot, F; Jeanne, N; Carcenac, R; Lefebvre, C; Izopet, J; French National Agency for Research on, Aids ; Viral Hepatitis, Resistance Study Group A C

Impact of the mutational load on the virological response to a first-line rilpivirine-based regimen Journal Article

J Antimicrob Chemother, 74 (3), pp. 718-721, 2019, ISSN: 1460-2091 (Electronic) 0305-7453 (Linking).

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Guerveno, C; Raymond, S; Metsu, D; Gandia, P; Izopet, J; Martin-Blondel, G; Delobel, P

HIV-1 escape in the central nervous system on elvitegravir-based antiretroviral therapy Journal Article

AIDS, 33 (3), pp. 593-594, 2019, ISSN: 1473-5571 (Electronic) 0269-9370 (Linking).

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Lhomme, S; DebRoy, S; Kamar, N; Abravanel, F; Metsu, D; Marion, O; Dimeglio, C; Cotler, S J; Izopet, J; Dahari, H

Plasma Hepatitis E Virus Kinetics in Solid Organ Transplant Patients Receiving Ribavirin Journal Article

Viruses, 11 (7), 2019, ISSN: 1999-4915 (Electronic) 1999-4915 (Linking).

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Lhomme, S; Gallian, P; Dimeglio, C; Assal, A; Abravanel, F; Tiberghien, P; Izopet, J

Viral load and clinical manifestations of hepatitis E virus genotype 3 infections Journal Article

J Viral Hepat, 26 (9), pp. 1139-1142, 2019, ISSN: 1365-2893 (Electronic) 1352-0504 (Linking).

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Loiseau, C; Requena, M; Nayrac, M; Mavigner, M; Cazabat, M; Iscache, A L; Carrere, N; Suc, B; Alric, L; Izopet, J; Delobel, P

Increased CXCR3+ T Cells Impairs Recruitment of T-Helper Type 17 Cells via Interferon gamma and Interleukin 18 in the Small Intestine Mucosa During Treated HIV-1 Infection Journal Article

J Infect Dis, 220 (5), pp. 830-840, 2019, ISSN: 1537-6613 (Electronic) 0022-1899 (Linking).

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Marion, O; Capelli, N; Lhomme, S; Dubois, M; Pucelle, M; Abravanel, F; Kamar, N; Izopet, J

Hepatitis E virus genotype 3 and capsid protein in the blood and urine of immunocompromised patients Journal Article

J Infect, 78 (3), pp. 232-240, 2019, ISSN: 1532-2742 (Electronic) 0163-4453 (Linking).

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Mzoughi, O; Teixido, M; Planes, R; Serrero, M; Hamimed, I; Zurita, E; Moreno, M; Granados, G; Lakhdar-Ghazal, F; BenMohamed, L; Giralt, E; Bahraoui, E

Trimeric Heptad Repeat Synthetic Peptides HR1 and HR2 Efficiently Inhibit HIV-1 Entry Journal Article

Biosci Rep, 2019, ISSN: 1573-4935 (Electronic) 0144-8463 (Linking).

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Sayed, I M; Verhoye, L; Montpellier, C; Abravanel, F; Izopet, J; Cocquerel, L; Meuleman, P

Hepatitis E Virus (HEV) Open Reading Frame 2 Antigen Kinetics in Human-Liver Chimeric Mice and Its Impact on HEV Diagnosis Journal Article

J Infect Dis, 220 (5), pp. 811-819, 2019, ISSN: 1537-6613 (Electronic) 0022-1899 (Linking).

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Dukhovny, A; Lamkiewicz, K; Chen, Q; Fricke, M; Jabrane-Ferrat, N; Marz, M; Jung, J U; Sklan, E H

A CRISPR Activation Screen Identifies Genes That Protect against Zika Virus Infection Journal Article

J Virol, 93 (16), 2019, ISSN: 1098-5514 (Electronic) 0022-538X (Linking).

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Hocine, H R; Brunel, S; Chen, Q; Giustiniani, J; San Roman, M J; Ferrat, Y J; Palacios, I; de la Rosa, O; Lombardo, E; Bensussan, A; Charron, D; Jabrane-Ferrat, N; Al-Daccak, R

Extracellular Vesicles Released by Allogeneic Human Cardiac Stem/Progenitor Cells as Part of Their Therapeutic Benefit Journal Article

Stem Cells Transl Med, 8 (9), pp. 911-924, 2019, ISSN: 2157-6580 (Electronic) 2157-6564 (Linking).

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Jabrane-Ferrat, N

Features of Human Decidual NK Cells in Healthy Pregnancy and During Viral Infection Journal Article

Front Immunol, 10 , pp. 1397, 2019, ISSN: 1664-3224 (Electronic) 1664-3224 (Linking).

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Mansuy, J M; El Costa, H; Gouilly, J; Mengelle, C; Pasquier, C; Martin-Blondel, G; Izopet, J; Jabrane-Ferrat, N

Peripheral Plasma and Semen Cytokine Response to Zika Virus in Humans Journal Article

Emerg Infect Dis, 25 (4), pp. 823-825, 2019, ISSN: 1080-6059 (Electronic) 1080-6040 (Linking).

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Souriant, S; Balboa, L; Dupont, M; Pingris, K; Kviatcovsky, D; Cougoule, C; Lastrucci, C; Bah, A; Gasser, R; Poincloux, R; Raynaud-Messina, B; Al Saati, T; Inwentarz, S; Poggi, S; Morana, E J; Gonzalez-Montaner, P; Corti, M; Lagane, B; Vergne, I; Allers, C; Kaushal, D; Kuroda, M J; Sasiain, M D C; Neyrolles, O; Maridonneau-Parini, I; Lugo-Villarino, G; Verollet, C

Tuberculosis Exacerbates HIV-1 Infection through IL-10/STAT3-Dependent Tunneling Nanotube Formation in Macrophages Journal Article

Cell Rep, 26 (13), pp. 3586-3599 e7, 2019, ISSN: 2211-1247 (Electronic).

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2018

Jin, J; Momboisse, F; Boncompain, G; Koensgen, F; Zhou, Z; Cordeiro, N; Arenzana-Seisdedos, F; Perez, F; Lagane, B; Kellenberger, E; Brelot, A

CCR5 adopts three homodimeric conformations that control cell surface delivery Journal Article

Sci Signal, 11 (529), 2018, ISSN: 1937-9145 (Electronic) 1945-0877 (Linking).

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Colin, P; Zhou, Z; Staropoli, I; Garcia-Perez, J; Gasser, R; Armani-Tourret, M; Benureau, Y; Gonzalez, N; Jin, J; Connell, B J; Raymond, S; Delobel, P; Izopet, J; Lortat-Jacob, H; Alcami, J; Arenzana-Seisdedos, F; Brelot, A; Lagane, B

CCR5 structural plasticity shapes HIV-1 phenotypic properties Journal Article

PLoS Pathog, 14 (12), pp. e1007432, 2018, ISSN: 1553-7374 (Electronic) 1553-7366 (Linking).

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Lopes, P P; Todorov, G; Pham, T T; Nesburn, A B; Bahraoui, E; BenMohamed, L

Laser Adjuvant-Assisted Peptide Vaccine Promotes Skin Mobilization of Dendritic Cells and Enhances Protective CD8(+) TEM and TRM Cell Responses against Herpesvirus Infection and Disease Journal Article

J Virol, 92 (8), 2018, ISSN: 1098-5514 (Electronic) 0022-538X (Linking).

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Srivastava, R; Hernandez-Ruiz, M; Khan, A A; Fouladi, M A; Kim, G J; Ly, V T; Yamada, T; Lam, C; Sarain, S A B; Boldbaatar, U; Zlotnik, A; Bahraoui, E; BenMohamed, L

CXCL17 Chemokine-Dependent Mobilization of CXCR8(+)CD8(+) Effector Memory and Tissue-Resident Memory T Cells in the Vaginal Mucosa Is Associated with Protection against Genital Herpes Journal Article

J Immunol, 200 (8), pp. 2915-2926, 2018, ISSN: 1550-6606 (Electronic) 0022-1767 (Linking).

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Planes, R; Serrero, M; Leghmari, K; BenMohamed, L; Bahraoui, E

HIV-1 Envelope Glycoproteins Induce the Production of TNF-alpha and IL-10 in Human Monocytes by Activating Calcium Pathway Journal Article

Sci Rep, 8 (1), pp. 17215, 2018, ISSN: 2045-2322 (Electronic) 2045-2322 (Linking).

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Halfon, P; Scholtes, C; Izopet, J; Larrat, S; Trimoulet, P; Zoulim, F; Alric, L; Metivier, S; Leroy, V; Ouzan, D; de Ledinghen, V; Mohamed, S; Penaranda, G; Khiri, H; Thelu, M A; Plauzolles, A; Chiche, L; Bourliere, M; Abravanel, F

Baseline and post-treatment hepatitis C NS5A resistance in relapsed patients from a multicentric real-life cohort Journal Article

Antivir Ther, 23 (4), pp. 307-314, 2018, ISSN: 2040-2058 (Electronic) 1359-6535 (Linking).

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Montpellier, C; Wychowski, C; Sayed, I M; Meunier, J C; Saliou, J M; Ankavay, M; Bull, A; Pillez, A; Abravanel, F; Helle, F; Brochot, E; Drobecq, H; Farhat, R; Aliouat-Denis, C M; Haddad, J G; Izopet, J; Meuleman, P; Goffard, A; Dubuisson, J; Cocquerel, L

Hepatitis E Virus Lifecycle and Identification of 3 Forms of the ORF2 Capsid Protein Journal Article

Gastroenterology, 154 (1), pp. 211-223 e8, 2018, ISSN: 1528-0012 (Electronic) 0016-5085 (Linking).

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Halfon, P; Scholtes, C; Izopet, J; Larrat, S; Trimoulet, P; Zoulim, F; Alric, L; Metivier, S; Leroy, V; Ouzan, D; de Ledinghen, V; Mohamed, S; Penaranda, G; Khiri, H; Thelu, M A; Plauzolles, A; Chiche, L; Bourliere, M; Abravanel, F

Retreatment with direct-acting antivirals of genotypes 1-3-4 hepatitis C patients who failed an anti-NS5A regimen in real world Journal Article

J Hepatol, 68 (3), pp. 595-597, 2018, ISSN: 1600-0641 (Electronic) 0168-8278 (Linking).

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Kamar, N; Del Bello, A; Izopet, J

Should Persistent Hepatitis E Virus Replication in Transplant Patients Be Tolerated? Journal Article

Transplantation, 102 (2), pp. e84-e85, 2018, ISSN: 1534-6080 (Electronic) 0041-1337 (Linking).

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Raymond, S; Nicot, F; Pallier, C; Bellecave, P; Maillard, A; Trabaud, M A; Morand-Joubert, L; Rodallec, A; Amiel, C; Mourez, T; Bocket, L; Beby-Defaux, A; Bouvier-Alias, M; Lambert-Niclot, S; Charpentier, C; Malve, B; Mirand, A; Dina, J; Le Guillou-Guillemette, H; Marque-Juillet, S; Signori-Schmuck, A; Barin, F; Si-Mohamed, A; Avettand Fenoel, V; Roussel, C; Calvez, V; Saune, K; Marcelin, A G; Rodriguez, C; Descamps, D; Izopet, J; French National Agency for Research on, Aids ; Viral Hepatitis, Resistance Study Group A C

Impact of Human Immunodeficiency Virus Type 1 Minority Variants on the Virus Response to a Rilpivirine-Based First-line Regimen Journal Article

Clin Infect Dis, 66 (10), pp. 1588-1594, 2018, ISSN: 1537-6591 (Electronic) 1058-4838 (Linking).

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Braun, P; Delgado, R; Drago, M; Fanti, D; Fleury, H; Izopet, J; Lombardi, A; Mancon, A; Marcos, M A; Saune, K; S, Shea O; Perez-Rivilla, A; Ramble, J; Trimoulet, P; Vila, J; Whittaker, D; Artus, A; Rhodes, D

A European multicenter study on the analytical performance of the VERIS HBV assay Journal Article

J Clin Virol, 99-100 , pp. 50-56, 2018, ISSN: 1873-5967 (Electronic) 1386-6532 (Linking).

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Raymond, S; Nicot, F; Carcenac, R; Lefebvre, C; Jeanne, N; Saune, K; Delobel, P; Izopet, J

HIV-1 genotypic resistance testing using the Vela automated next-generation sequencing platform Journal Article

J Antimicrob Chemother, 73 (5), pp. 1152-1157, 2018, ISSN: 1460-2091 (Electronic) 0305-7453 (Linking).

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Bisseux, M; Colombet, J; Mirand, A; Roque-Afonso, A M; Abravanel, F; Izopet, J; Archimbaud, C; Peigue-Lafeuille, H; Debroas, D; Bailly, J L; Henquell, C

Monitoring human enteric viruses in wastewater and relevance to infections encountered in the clinical setting: a one-year experiment in central France, 2014 to 2015 Journal Article

Euro Surveill, 23 (7), 2018, ISSN: 1560-7917 (Electronic) 1025-496X (Linking).

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Dalton, H R; Izopet, J

Transmission and Epidemiology of Hepatitis E Virus Genotype 3 and 4 Infections Journal Article

Cold Spring Harb Perspect Med, 8 (11), 2018, ISSN: 2157-1422 (Electronic) 2157-1422 (Linking).

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Mallet, V; Sberro-Soussan, R; Roque-Afonso, A M; Vallet-Pichard, A; Deau, B; Portal, A; Chaix, M L; Hauser, L; Beyloune, A; Mercadier, A; Izopet, J; Legendre, C; Pol, S

Transmission of Hepatitis E Virus With Plasma Exchange in Kidney Transplant Recipients: A Retrospective Cohort Study Journal Article

Transplantation, 102 (8), pp. 1351-1357, 2018, ISSN: 1534-6080 (Electronic) 0041-1337 (Linking).

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Kamar, N; Pischke, S

Acute and Persistent Hepatitis E Virus Genotype 3 and 4 Infection: Clinical Features, Pathogenesis, and Treatment Journal Article

Cold Spring Harb Perspect Med, 2018, ISSN: 2157-1422 (Electronic) 2157-1422 (Linking).

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Nicot, F; Jeanne, N; Raymond, S; Delfour, O; Carcenac, R; Lefebvre, C; Saune, K; Delobel, P; Izopet, J

Performance comparison of deep sequencing platforms for detecting HIV-1 variants in the pol gene Journal Article

J Med Virol, 90 (9), pp. 1486-1492, 2018, ISSN: 1096-9071 (Electronic) 0146-6615 (Linking).

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Lambert-Niclot, S; Grude, M; Meynard, J L; Marcelin, A G; Valantin, M A; Flandre, P; Izopet, J; Moinot, L; Bouteloup, V; Calvez, V; Katlama, C; Girard, P M; Morand-Joubert, L

Ultrasensitive Human Immunodeficiency Virus Type 1 Viral Load as a Marker of Treatment Choice for Simplification Strategies Journal Article

Clin Infect Dis, 67 (12), pp. 1883-1889, 2018, ISSN: 1537-6591 (Electronic) 1058-4838 (Linking).

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Grellier, J; Hirsch, H H; Mengelle, C; Esposito, L; Hebral, A L; Belliere, J; Weissbach, F; Izopet, J; Del Bello, A; Kamar, N

Impact of donor BK polyomavirus replication on recipient infections in living donor transplantation Journal Article

Transpl Infect Dis, 20 (4), pp. e12917, 2018, ISSN: 1399-3062 (Electronic) 1398-2273 (Linking).

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Marion, O; Abravanel, F; Del Bello, A; Esposito, L; Lhomme, S; Puissant-Lubrano, B; Alric, L; Faguer, S; Izopet, J; Kamar, N

Hepatitis E virus-associated cryoglobulinemia in solid-organ-transplant recipients Journal Article

Liver Int, 38 (12), pp. 2178-2189, 2018, ISSN: 1478-3231 (Electronic) 1478-3223 (Linking).

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Nicot, F; Jeanne, N; Roulet, A; Lefebvre, C; Carcenac, R; Manno, M; Dubois, M; Kamar, N; Lhomme, S; Abravanel, F; Izopet, J

Diversity of hepatitis E virus genotype 3 Journal Article

Rev Med Virol, 28 (5), pp. e1987, 2018, ISSN: 1099-1654 (Electronic) 1052-9276 (Linking).

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Abravanel, F; Lhomme, S; Chapuy-Regaud, S; Mansuy, J M; Boineau, J; Saune, K; Izopet, J

A fully automated system using transcription-mediated amplification for the molecular diagnosis of hepatitis E virus in human blood and faeces Journal Article

J Clin Virol, 105 , pp. 109-111, 2018, ISSN: 1873-5967 (Electronic) 1386-6532 (Linking).

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Abravanel, F; Pique, J; Couturier, E; Nicot, F; Dimeglio, C; Lhomme, S; Chiabrando, J; Saune, K; Peron, J M; Kamar, N; Evrard, S; de Valk, H; Cintas, P; Izopet, J; study , H E V

Acute hepatitis E in French patients and neurological manifestations Journal Article

J Infect, 77 (3), pp. 220-226, 2018, ISSN: 1532-2742 (Electronic) 0163-4453 (Linking).

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Mansuy, J M; Lhomme, S; Cazabat, M; Pasquier, C; Martin-Blondel, G; Izopet, J

Detection of Zika, dengue and chikungunya viruses using single-reaction multiplex real-time RT-PCR Journal Article

Diagn Microbiol Infect Dis, 92 (4), pp. 284-287, 2018, ISSN: 1879-0070 (Electronic) 0732-8893 (Linking).

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Raymond, S; Delaugerre, C; Nicot, F; Assoumou, L; Lancar, R; Beniguel, L; Izopet, J; study , Anrs Optimal

Accurate quantification of CXCR4-using HIV-1 variants by Illumina deep-sequencing Journal Article

AIDS, 32 (16), pp. 2429-2431, 2018, ISSN: 1473-5571 (Electronic) 0269-9370 (Linking).

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Jadoul, M; Berenguer, M C; Doss, W; Fabrizi, F; Izopet, J; Jha, V; Kamar, N; Kasiske, B L; Lai, C L; Morales, J M; Patel, P R; Pol, S; Silva, M O; Balk, E M; Gordon, C E; Earley, A; Di, M; Martin, P

Executive summary of the 2018 KDIGO Hepatitis C in CKD Guideline: welcoming advances in evaluation and management Journal Article

Kidney Int, 94 (4), pp. 663-673, 2018, ISSN: 1523-1755 (Electronic) 0085-2538 (Linking).

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Abravanel, F; Lacipiere, A; Lhomme, S; Dubois, M; Minier, L; Peron, J M; Alric, L; Kamar, N; Izopet, J

Performance of a commercial assay for detecting and quantifying HEV RNA in faeces Journal Article

J Clin Virol, 109 , pp. 1-5, 2018, ISSN: 1873-5967 (Electronic) 1386-6532 (Linking).

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Capelli, N; Marion, O; Dubois, M; Allart, S; Bertrand-Michel, J; Lhomme, S; Abravanel, F; Izopet, J; Chapuy-Regaud, S

Vectorial Release of Hepatitis E Virus in Polarized Human Hepatocytes Journal Article

J Virol, 2018, ISSN: 1098-5514 (Electronic) 0022-538X (Linking).

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Dimeglio, C; Beau, F; Broult, J; Gouy, P; Izopet, J; Lastere, S; Abravanel, F

Hepatitis E prevalence in French Polynesian blood donors Journal Article

PLoS One, 13 (12), pp. e0208934, 2018, ISSN: 1932-6203 (Electronic) 1932-6203 (Linking).

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Dimeglio, C; Raymond, S; Nicot, F; Jeanne, N; Carcenac, R; Lefebvre, C; Izopet, J; French National Agency for Research on, Aids ; Viral Hepatitis, Resistance Study Group A C

Impact of the mutational load on the virological response to a first-line rilpivirine-based regimen Journal Article

J Antimicrob Chemother, 2018, ISSN: 1460-2091 (Electronic) 0305-7453 (Linking).

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Gouilly, J; Chen, Q; Siewiera, J; Cartron, G; Levy, C; Dubois, M; Al-Daccak, R; Izopet, J; Jabrane-Ferrat, N; El Costa, H

Genotype specific pathogenicity of hepatitis E virus at the human maternal-fetal interface Journal Article

Nat Commun, 9 (1), pp. 4748, 2018, ISSN: 2041-1723 (Electronic) 2041-1723 (Linking).

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2017

Lhomme, S; Bardiaux, L; Abravanel, F; Gallian, P; Kamar, N; Izopet, J

Hepatitis E Virus Infection in Solid Organ Transplant Recipients, France Journal Article

Emerg Infect Dis, 23 (2), pp. 353-356, 2017, ISSN: 1080-6059 (Electronic) 1080-6040 (Linking).

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Srivastava, R; Khan, A A; Chilukuri, S; Syed, S A; Tran, T T; Furness, J; Bahraoui, E; BenMohamed, L

CXCL10/CXCR3-Dependent Mobilization of Herpes Simplex Virus-Specific CD8(+) TEM and CD8(+) TRM Cells within Infected Tissues Allows Efficient Protection against Recurrent Herpesvirus Infection and Disease Journal Article

J Virol, 91 (14), 2017, ISSN: 1098-5514 (Electronic) 0022-538X (Linking).

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Serrero, M; Planes, R; Bahraoui, E

PKC-delta isoform plays a crucial role in Tat-TLR4 signalling pathway to activate NF-kappaB and CXCL8 production Journal Article

Sci Rep, 7 (1), pp. 2384, 2017, ISSN: 2045-2322 (Electronic) 2045-2322 (Linking).

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Donnelly, M C; Imlach, S N; Abravanel, F; Ramalingam, S; Johannessen, I; Petrik, J; Fraser, A R; Campbell, J D; Bramley, P; Dalton, H R; Hayes, P C; Kamar, N; Simpson, K J

Sofosbuvir and Daclatasvir Anti-Viral Therapy Fails to Clear HEV Viremia and Restore Reactive T Cells in a HEV/HCV Co-Infected Liver Transplant Recipient Journal Article

Gastroenterology, 152 (1), pp. 300-301, 2017, ISSN: 1528-0012 (Electronic) 0016-5085 (Linking).

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Abravanel, F; Lhomme, S; Fougere, M; Saune, K; Alvarez, M; Peron, J M; Delobel, P; Izopet, J

HEV infection in French HIV-infected patients Journal Article

J Infect, 74 (3), pp. 310-313, 2017, ISSN: 1532-2742 (Electronic) 0163-4453 (Linking).

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Raymond, S; Nicot, F; Jeanne, N; Delfour, O; Carcenac, R; Lefebvre, C; Cazabat, M; Saune, K; Delobel, P; Izopet, J

Performance comparison of next-generation sequencing platforms for determining HIV-1 coreceptor use Journal Article

Sci Rep, 7 , pp. 42215, 2017, ISSN: 2045-2322 (Electronic) 2045-2322 (Linking).

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Sayed, I M; Foquet, L; Verhoye, L; Abravanel, F; Farhoudi, A; Leroux-Roels, G; Izopet, J; Meuleman, P

Transmission of hepatitis E virus infection to human-liver chimeric FRG mice using patient plasma Journal Article

Antiviral Res, 141 , pp. 150-154, 2017, ISSN: 1872-9096 (Electronic) 0166-3542 (Linking).

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Abravanel, F; Goutagny, N; Perret, C; Lhomme, S; Vischi, F; Aversenq, A; Chapel, A; Dehainault, N; Piga, N; Dupret-Carruel, J; Izopet, J

Evaluation of two VIDAS ((R))prototypes for detecting anti-HEV IgG Journal Article

J Clin Virol, 89 , pp. 46-50, 2017, ISSN: 1873-5967 (Electronic) 1386-6532 (Linking).

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Mansuy, J M; Mengelle, C; Pasquier, C; Chapuy-Regaud, S; Delobel, P; Martin-Blondel, G; Izopet, J

Zika Virus Infection and Prolonged Viremia in Whole-Blood Specimens Journal Article

Emerg Infect Dis, 23 (5), pp. 863-865, 2017, ISSN: 1080-6059 (Electronic) 1080-6040 (Linking).

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Pasquier, C; Saune, K; Raymond, S; Boisneau, J; Courtade, M; Izopet, J

Comparison of Cobas(R) HPV and Anyplex II HPV28 assays for detecting and genotyping human papillomavirus Journal Article

Diagn Microbiol Infect Dis, 87 (1), pp. 25-27, 2017, ISSN: 1879-0070 (Electronic) 0732-8893 (Linking).

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Kamar, N; Wang, W; Dalton, H R; Pan, Q

Direct-acting antiviral therapy for hepatitis E virus? Journal Article

Lancet Gastroenterol Hepatol, 2 (3), pp. 154-155, 2017, ISSN: 2468-1253 (Electronic).

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Velay, A; Kack-Kack, W; Abravanel, F; Lhomme, S; Leyendecker, P; Kremer, L; Chamouard, P; Izopet, J; Fafi-Kremer, S; Barth, H

Parsonage-Turner syndrome due to autochthonous acute genotype 3f hepatitis E virus infection in a nonimmunocompromised 55-year-old patient Journal Article

J Neurovirol, 23 (4), pp. 615-620, 2017, ISSN: 1538-2443 (Electronic) 1355-0284 (Linking).

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Chapuy-Regaud, S; Dubois, M; Plisson-Chastang, C; Bonnefois, T; Lhomme, S; Bertrand-Michel, J; You, B; Simoneau, S; Gleizes, P E; Flan, B; Abravanel, F; Izopet, J

Characterization of the lipid envelope of exosome encapsulated HEV particles protected from the immune response Journal Article

Biochimie, 141 , pp. 70-79, 2017, ISSN: 1638-6183 (Electronic) 0300-9084 (Linking).

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Dorr, G; Del Bello, A; Abravanel, F; Marion, O; Cointault, O; Ribes, D; Lavayssiere, L; Esposito, L; Nogier, M B; Hebral, A L; Saune, K; Izopet, J; Kamar, N

Malignancies in hepatitis C virus-positive and -negative kidney transplant recipients: A case-controlled study Journal Article

Transpl Infect Dis, 19 (4), 2017, ISSN: 1399-3062 (Electronic) 1398-2273 (Linking).

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Yin, X; Li, X; Ambardekar, C; Hu, Z; Lhomme, S; Feng, Z

Hepatitis E virus persists in the presence of a type III interferon response Journal Article

PLoS Pathog, 13 (5), pp. e1006417, 2017, ISSN: 1553-7374 (Electronic) 1553-7366 (Linking).

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Metsu, D; Toutain, P L; Chatelut, E; Delobel, P; Gandia, P

Antiretroviral unbound concentration during pregnancy: piece of interest in the puzzle? Journal Article

J Antimicrob Chemother, 72 (9), pp. 2407-2409, 2017, ISSN: 1460-2091 (Electronic) 0305-7453 (Linking).

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Abravanel, F; Lhomme, S; El Costa, H; Schvartz, B; Peron, J M; Kamar, N; Izopet, J

Rabbit Hepatitis E Virus Infections in Humans, France Journal Article

Emerg Infect Dis, 23 (7), pp. 1191-1193, 2017, ISSN: 1080-6059 (Electronic) 1080-6040 (Linking).

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Moulin, M; Alguacil, J; Gu, S; Mehtougui, A; Adams, E J; Peyrottes, S; Champagne, E

Vgamma9Vdelta2 T cell activation by strongly agonistic nucleotidic phosphoantigens Journal Article

Cell Mol Life Sci, 74 (23), pp. 4353-4367, 2017, ISSN: 1420-9071 (Electronic) 1420-682X (Linking).

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Izopet, J; Lhomme, S; Chapuy-Regaud, S; Mansuy, J M; Kamar, N; Abravanel, F

HEV and transfusion-recipient risk Journal Article

Transfus Clin Biol, 24 (3), pp. 176-181, 2017, ISSN: 1953-8022 (Electronic) 1246-7820 (Linking).

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Dalton, H R; van Eijk, J J J; Cintas, P; Madden, R G; Jones, C; Webb, G W; Norton, B; Pique, J; Lutgens, S; Devooght-Johnson, N; Woolson, K; Baker, J; Saunders, M; Househam, L; Griffiths, J; Abravanel, F; Izopet, J; Kamar, N; van Alfen, N; van Engelen, B G M; Hunter, J G; van der Eijk, A A; Bendall, R P; McLean, B N; Jacobs, B C

Hepatitis E virus infection and acute non-traumatic neurological injury: A prospective multicentre study Journal Article

J Hepatol, 67 (5), pp. 925-932, 2017, ISSN: 1600-0641 (Electronic) 0168-8278 (Linking).

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Joguet, G; Mansuy, J M; Matusali, G; Hamdi, S; Walschaerts, M; Pavili, L; Guyomard, S; Prisant, N; Lamarre, P; Dejucq-Rainsford, N; Pasquier, C; Bujan, L

Effect of acute Zika virus infection on sperm and virus clearance in body fluids: a prospective observational study Journal Article

Lancet Infect Dis, 17 (11), pp. 1200-1208, 2017, ISSN: 1474-4457 (Electronic) 1473-3099 (Linking).

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Pasquier, C; Walschaerts, M; Raymond, S; Moinard, N; Saune, K; Daudin, M; Izopet, J; Bujan, L

Patterns of residual HIV-1 RNA shedding in the seminal plasma of patients on effective antiretroviral therapy Journal Article

Basic Clin Androl, 27 , pp. 17, 2017, ISSN: 2051-4190 (Print) 2051-4190 (Linking).

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Barrague, H; Condat, B; Petitdidier, N; Champagne, E; Renou, C; Izopet, J; Abravanel, F

Chronic hepatitis E virus infection in a cirrhotic patient: A case report Journal Article

Medicine (Baltimore), 96 (39), pp. e7915, 2017, ISSN: 1536-5964 (Electronic) 0025-7974 (Linking).

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Belliere, J; Abravanel, F; Nogier, M B; Martinez, S; Cintas, P; Lhomme, S; Lavayssiere, L; Cointault, O; Faguer, S; Izopet, J; Kamar, N

Transfusion-acquired hepatitis E infection misdiagnosed as severe critical illness polyneuromyopathy in a heart transplant patient Journal Article

Transpl Infect Dis, 19 (6), 2017, ISSN: 1399-3062 (Electronic) 1398-2273 (Linking).

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Kamar, N; Izopet, J; Pavio, N; Aggarwal, R; Labrique, A; Wedemeyer, H; Dalton, H R

Hepatitis E virus infection Journal Article

Nat Rev Dis Primers, 3 , pp. 17086, 2017, ISSN: 2056-676X (Electronic) 2056-676X (Linking).

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de Truchis, P; Assoumou, L; Landman, R; Mathez, D; Le Du, D; Bellet, J; Amat, K; Katlama, C; Gras, G; Bouchaud, O; Duracinsky, M; Abe, E; Alvarez, J C; Izopet, J; Saillard, J; Melchior, J C; Leibowitch, J; Costagliola, D; Girard, P M; Perronne, C; Group, Anrs Study D

Four-days-a-week antiretroviral maintenance therapy in virologically controlled HIV-1-infected adults: the ANRS 162-4D trial Journal Article

J Antimicrob Chemother, 2017, ISSN: 1460-2091 (Electronic) 0305-7453 (Linking).

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Hocine, H R; Costa, H E; Dam, N; Giustiniani, J; Palacios, I; Loiseau, P; Bensussan, A; Borlado, L R; Charron, D; Suberbielle, C; Jabrane-Ferrat, N; Al-Daccak, R

Minimizing the risk of allo-sensitization to optimize the benefit of allogeneic cardiac-derived stem/progenitor cells Journal Article

Sci Rep, 7 , pp. 41125, 2017, ISSN: 2045-2322 (Electronic) 2045-2322 (Linking).

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Dam, N; Hocine, H R; Palacios, I; DelaRosa, O; Menta, R; Charron, D; Bensussan, A; El Costa, H; Jabrane-Ferrat, N; Dalemans, W; Lombardo, E; Al-Daccak, R

Human Cardiac-Derived Stem/Progenitor Cells Fine-Tune Monocyte-Derived Descendants Activities toward Cardiac Repair Journal Article

Front Immunol, 8 , pp. 1413, 2017, ISSN: 1664-3224 (Print) 1664-3224 (Linking).

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2016

Abravanel, F; Barrague, H; Dorr, G; Saune, K; Peron, J M; Alric, L; Kamar, N; Izopet, J; Champagne, E

Conventional and innate lymphocytes response at the acute phase of HEV infection in transplanted patients Journal Article

J Infect, 72 (6), pp. 723-730, 2016, ISSN: 1532-2742 (Electronic) 0163-4453 (Linking).

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Gasser, R; Hamoudi, M; Pellicciotta, M; Zhou, Z; Visdeloup, C; Colin, P; Braibant, M; Lagane, B; Negroni, M

Buffering deleterious polymorphisms in highly constrained parts of HIV-1 envelope by flexible regions Journal Article

Retrovirology, 13 (1), pp. 50, 2016, ISSN: 1742-4690 (Electronic) 1742-4690 (Linking).

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Loiseau, C; Requena, M; Mavigner, M; Cazabat, M; Carrere, N; Suc, B; Barange, K; Alric, L; Marchou, B; Massip, P; Izopet, J; Delobel, P

CCR6(-) regulatory T cells blunt the restoration of gut Th17 cells along the CCR6-CCL20 axis in treated HIV-1-infected individuals Journal Article

Mucosal Immunol, 9 (5), pp. 1137-50, 2016, ISSN: 1935-3456 (Electronic) 1933-0219 (Linking).

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Mansuy, J M; Dutertre, M; Mengelle, C; Fourcade, C; Marchou, B; Delobel, P; Izopet, J; Martin-Blondel, G

Zika virus: high infectious viral load in semen, a new sexually transmitted pathogen? Journal Article

Lancet Infect Dis, 16 (4), pp. 405, 2016, ISSN: 1474-4457 (Electronic) 1473-3099 (Linking).

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Benureau, Y; Colin, P; Staropoli, I; Gonzalez, N; Garcia-Perez, J; Alcami, J; Arenzana-Seisdedos, F; Lagane, B

Guidelines for cloning, expression, purification and functional characterization of primary HIV-1 envelope glycoproteins Journal Article

J Virol Methods, 236 , pp. 184-95, 2016, ISSN: 1879-0984 (Electronic) 0166-0934 (Linking).

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Planes, R; Ben Haij, N; Leghmari, K; Serrero, M; BenMohamed, L; Bahraoui, E

HIV-1 Tat Protein Activates both the MyD88 and TRIF Pathways To Induce Tumor Necrosis Factor Alpha and Interleukin-10 in Human Monocytes Journal Article

J Virol, 90 (13), pp. 5886-5898, 2016, ISSN: 1098-5514 (Electronic) 0022-538X (Linking).

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El Costa, H; Gouilly, J; Mansuy, J M; Chen, Q; Levy, C; Cartron, G; Veas, F; Al-Daccak, R; Izopet, J; Jabrane-Ferrat, N

ZIKA virus reveals broad tissue and cell tropism during the first trimester of pregnancy Journal Article

Sci Rep, 6 , pp. 35296, 2016, ISSN: 2045-2322 (Electronic) 2045-2322 (Linking).

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2015

Garcia-Perez, J; Staropoli, I; Azoulay, S; Heinrich, J T; Cascajero, A; Colin, P; Lortat-Jacob, H; Arenzana-Seisdedos, F; Alcami, J; Kellenberger, E; Lagane, B

A single-residue change in the HIV-1 V3 loop associated with maraviroc resistance impairs CCR5 binding affinity while increasing replicative capacity Journal Article

Retrovirology, 12 , pp. 50, 2015, ISSN: 1742-4690 (Electronic) 1742-4690 (Linking).

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Jeanne, N; Saliou, A; Carcenac, R; Lefebvre, C; Dubois, M; Cazabat, M; Nicot, F; Loiseau, C; Raymond, S; Izopet, J; Delobel, P

Position-specific automated processing of V3 env ultra-deep pyrosequencing data for predicting HIV-1 tropism Journal Article

Sci Rep, 5 , pp. 16944, 2015, ISSN: 2045-2322 (Electronic) 2045-2322 (Linking).

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Lhomme, S; Kamar, N; Nicot, F; Ducos, J; Bismuth, M; Garrigue, V; Petitjean-Lecherbonnier, J; Ollivier, I; Alessandri-Gradt, E; Goria, O; Barth, H; Perrin, P; Saune, K; Dubois, M; Carcenac, R; Lefebvre, C; Jeanne, N; Abravanel, F; Izopet, J

Mutation in the Hepatitis E Virus Polymerase and Outcome of Ribavirin Therapy Journal Article

Antimicrob Agents Chemother, 60 (3), pp. 1608-14, 2015, ISSN: 1098-6596 (Electronic) 0066-4804 (Linking).

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Ben Haij, N; Planes, R; Leghmari, K; Serrero, M; Delobel, P; Izopet, J; BenMohamed, L; Bahraoui, E

HIV-1 Tat Protein Induces Production of Proinflammatory Cytokines by Human Dendritic Cells and Monocytes/Macrophages through Engagement of TLR4-MD2-CD14 Complex and Activation of NF-kappaB Pathway Journal Article

PLoS One, 10 (6), pp. e0129425, 2015, ISSN: 1932-6203 (Electronic) 1932-6203 (Linking).

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Ainaoui, N; Hantelys, F; Renaud-Gabardos, E; Bunel, M; Lopez, F; Pujol, F; Planes, R; Bahraoui, E; Pichereaux, C; Burlet-Schiltz, O; Parini, A; Garmy-Susini, B; Prats, A C

Promoter-Dependent Translation Controlled by p54nrb and hnRNPM during Myoblast Differentiation Journal Article

PLoS One, 10 (9), pp. e0136466, 2015, ISSN: 1932-6203 (Electronic) 1932-6203 (Linking).

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Siewiera, J; Gouilly, J; Hocine, H R; Cartron, G; Levy, C; Al-Daccak, R; Jabrane-Ferrat, N

Natural cytotoxicity receptor splice variants orchestrate the distinct functions of human natural killer cell subtypes Journal Article

Nat Commun, 6 , pp. 10183, 2015, ISSN: 2041-1723 (Electronic) 2041-1723 (Linking).

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2014

Kamar, N; Izopet, J; Tripon, S; Bismuth, M; Hillaire, S; Dumortier, J; Radenne, S; Coilly, A; Garrigue, V; D'Alteroche, L; Buchler, M; Couzi, L; Lebray, P; Dharancy, S; Minello, A; Hourmant, M; Roque-Afonso, A M; Abravanel, F; Pol, S; Rostaing, L; Mallet, V

Ribavirin for chronic hepatitis E virus infection in transplant recipients Journal Article

N Engl J Med, 370 (12), pp. 1111-20, 2014, ISSN: 1533-4406 (Electronic) 0028-4793 (Linking).

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Planes, R; BenMohamed, L; Leghmari, K; Delobel, P; Izopet, J; Bahraoui, E

HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms Journal Article

J Virol, 88 (12), pp. 6672-89, 2014, ISSN: 1098-5514 (Electronic) 0022-538X (Linking).

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Bahraoui, E; Briant, L; Chazal, N

E5564 inhibits immunosuppressive cytokine IL-10 induction promoted by HIV-1 Tat protein Journal Article

Virol J, 11 , pp. 214, 2014, ISSN: 1743-422X (Electronic) 1743-422X (Linking).

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Jabrane-Ferrat, N; Siewiera, J

The up side of decidual natural killer cells: new developments in immunology of pregnancy Journal Article

Immunology, 141 (4), pp. 490-7, 2014, ISSN: 1365-2567 (Electronic) 0019-2805 (Linking).

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Lauden, L; Siewiera, J; Boukouaci, W; Ramgolam, K; Mourah, S; Lebbe, C; Charron, D; Aoudjit, F; Jabrane-Ferrat, N; Al-Daccak, R

TGF-beta-induced (TGFBI) protein in melanoma: a signature of high metastatic potential Journal Article

J Invest Dermatol, 134 (6), pp. 1675-1685, 2014, ISSN: 1523-1747 (Electronic) 0022-202X (Linking).

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Boukouaci, W; Lauden, L; Siewiera, J; Dam, N; Hocine, H R; Khaznadar, Z; Tamouza, R; Borlado, L R; Charron, D; Jabrane-Ferrat, N; Al-Daccak, R

Natural killer cell crosstalk with allogeneic human cardiac-derived stem/progenitor cells controls persistence Journal Article

Cardiovasc Res, 104 (2), pp. 290-302, 2014, ISSN: 1755-3245 (Electronic) 0008-6363 (Linking).

Links | BibTeX

Social impact

Knowledge of the biology of RNA viruses and pathophysiological mechanisms.

Our research generates fundamental knowledge about chronic viral infections (HIV and HEV) and emerging infection (ZIKV), and clinical applications to combat these viruses whose pathophysiology remains unclear. Our links with the clinical departments of Toulouse University Hospital, with the technical platform of the CPTP and several international collaborations ensure the optimal development of original themes.

Teaching commitment

Our team members are committed to the teaching of students in the faculties of Medicine and Pharmacy; we also welcome Master and PhD students from both France and abroad to work and study in our laboratory.

Alumni

PhD students:

Maud Mavigner (2008-2011)
Nawal Benhaji (2009-2013)
Adrien Saliou (2010-2013)
Johan Siewiera (2010-2014)
Claire Loiseau (2012-2015)
Charline Daguzan (2012-2015)
Hugo Barragué (2015-2018)
Jordi Gouilly (2015-2018)
Manon Nayrac (2015-2019)
Nicolas Capelli (2016-2020)
Qian Chen (2016-2020)

Post Doctoral Fellow:

Sokunthea Top (2013-2014)
Romain Gasser (2016-2018)

INSERM

INSERM

CNRS

CNRS

ANRS

ANRS

Sidaction

Sidaction

Hôpitaux de Toulouse

Hôpitaux de Toulouse

Agence de la biomédecine

Agence de la biomédecine

UT3

UT3