Pelletier, L.; Savignac, M. Ca(2+) signaling in T-cell subsets with a focus on the role of cav1 channels: possible implications in therapeutics Journal Article In: Front Immunol, vol. 4, pp. 150, 2013, ISSN: 1664-3224 (Print)
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Robert, V.; Triffaux, E.; Savignac, M.; Pelletier, L. Singularities of calcium signaling in effector T-lymphocytes Journal Article In: Biochim Biophys Acta, vol. 1833, no. 7, pp. 1595-602, 2013, ISSN: 0006-3002 (Print)
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Seillet, C.; Rouquie, N.; Foulon, E.; Douin-Echinard, V.; Krust, A.; Chambon, P.; Arnal, J. F.; Guery, J. C.; Laffont, S. Estradiol Promotes Functional Responses in Inflammatory and Steady-State Dendritic Cells through Differential Requirement for Activation Function-1 of Estrogen Receptor alpha Journal Article In: J. Immunol., vol. 190, no. 11, pp. 5459-70, 2013, ISSN: 1550-6606 (Electronic)
0022-1767 (Linking). @article{RN10b,
title = {Estradiol Promotes Functional Responses in Inflammatory and Steady-State Dendritic Cells through Differential Requirement for Activation Function-1 of Estrogen Receptor alpha},
author = {Seillet, C. and Rouquie, N. and Foulon, E. and Douin-Echinard, V. and Krust, A. and Chambon, P. and Arnal, J. F. and Guery, J. C. and Laffont, S.},
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Ariey, F.; Witkowski, B.; Amaratunga, C.; Beghain, J.; Langlois, A. C.; Khim, N.; Kim, S.; Duru, V.; Bouchier, C.; Ma, L.; Lim, P.; Leang, R.; Duong, S.; Sreng, S.; Suon, S.; Chuor, C. M.; Bout, D. M.; Menard, S.; Rogers, W. O.; Genton, B.; Fandeur, T.; Miotto, O.; Ringwald, P.; Le Bras, J.; Berry, A.; Barale, J. C.; Fairhurst, R. M.; Benoit-Vical, F.; Mercereau-Puijalon, O.; Menard, D. A molecular marker of artemisinin-resistant Plasmodium falciparum malaria Journal Article In: Nature, 2013, (Ariey, Frederic
Witkowski, Benoit
Amaratunga, Chanaki
Beghain, Johann
Langlois, Anne-Claire
Khim, Nimol
Kim, Saorin
Duru, Valentine
Bouchier, Christiane
Ma, Laurence
Lim, Pharath
Leang, Rithea
Duong, Socheat
Sreng, Sokunthea
Suon, Seila
Chuor, Char Meng
Bout, Denis Mey
Menard, Sandie
Rogers, William O
Genton, Blaise
Fandeur, Thierry
Miotto, Olivo
Ringwald, Pascal
Le Bras, Jacques
Berry, Antoine
Barale, Jean-Christophe
Fairhurst, Rick M
Benoit-Vical, Francoise
Mercereau-Puijalon, Odile
Menard, Didier
Nature. 2013 Dec 18. doi: 10.1038/nature12876.). @article{b,
title = {A molecular marker of artemisinin-resistant Plasmodium falciparum malaria},
author = {Ariey, F. and Witkowski, B. and Amaratunga, C. and Beghain, J. and Langlois, A. C. and Khim, N. and Kim, S. and Duru, V. and Bouchier, C. and Ma, L. and Lim, P. and Leang, R. and Duong, S. and Sreng, S. and Suon, S. and Chuor, C. M. and Bout, D. M. and Menard, S. and Rogers, W. O. and Genton, B. and Fandeur, T. and Miotto, O. and Ringwald, P. and Le Bras, J. and Berry, A. and Barale, J. C. and Fairhurst, R. M. and Benoit-Vical, F. and Mercereau-Puijalon, O. and Menard, D.},
year = {2013},
date = {2013-01-01},
journal = {Nature},
abstract = {Plasmodium falciparum resistance to artemisinin derivatives in southeast Asia threatens malaria control and elimination activities worldwide. To monitor the spread of artemisinin resistance, a molecular marker is urgently needed. Here, using whole-genome sequencing of an artemisinin-resistant parasite line from Africa and clinical parasite isolates from Cambodia, we associate mutations in the PF3D7_1343700 kelch propeller domain ('K13-propeller') with artemisinin resistance in vitro and in vivo. Mutant K13-propeller alleles cluster in Cambodian provinces where resistance is prevalent, and the increasing frequency of a dominant mutant K13-propeller allele correlates with the recent spread of resistance in western Cambodia. Strong correlations between the presence of a mutant allele, in vitro parasite survival rates and in vivo parasite clearance rates indicate that K13-propeller mutations are important determinants of artemisinin resistance. K13-propeller polymorphism constitutes a useful molecular marker for large-scale surveillance efforts to contain artemisinin resistance in the Greater Mekong Subregion and prevent its global spread.},
note = {Ariey, Frederic
Witkowski, Benoit
Amaratunga, Chanaki
Beghain, Johann
Langlois, Anne-Claire
Khim, Nimol
Kim, Saorin
Duru, Valentine
Bouchier, Christiane
Ma, Laurence
Lim, Pharath
Leang, Rithea
Duong, Socheat
Sreng, Sokunthea
Suon, Seila
Chuor, Char Meng
Bout, Denis Mey
Menard, Sandie
Rogers, William O
Genton, Blaise
Fandeur, Thierry
Miotto, Olivo
Ringwald, Pascal
Le Bras, Jacques
Berry, Antoine
Barale, Jean-Christophe
Fairhurst, Rick M
Benoit-Vical, Francoise
Mercereau-Puijalon, Odile
Menard, Didier
Nature. 2013 Dec 18. doi: 10.1038/nature12876.},
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Plasmodium falciparum resistance to artemisinin derivatives in southeast Asia threatens malaria control and elimination activities worldwide. To monitor the spread of artemisinin resistance, a molecular marker is urgently needed. Here, using whole-genome sequencing of an artemisinin-resistant parasite line from Africa and clinical parasite isolates from Cambodia, we associate mutations in the PF3D7_1343700 kelch propeller domain ('K13-propeller') with artemisinin resistance in vitro and in vivo. Mutant K13-propeller alleles cluster in Cambodian provinces where resistance is prevalent, and the increasing frequency of a dominant mutant K13-propeller allele correlates with the recent spread of resistance in western Cambodia. Strong correlations between the presence of a mutant allele, in vitro parasite survival rates and in vivo parasite clearance rates indicate that K13-propeller mutations are important determinants of artemisinin resistance. K13-propeller polymorphism constitutes a useful molecular marker for large-scale surveillance efforts to contain artemisinin resistance in the Greater Mekong Subregion and prevent its global spread. |
Feliu, V.; Vasseur, V.; Grover, H. S.; Chu, H. H.; Brown, M. J.; Wang, J.; Boyle, J. P.; Robey, E. A.; Shastri, N.; Blanchard, N. Location of the CD8 T Cell Epitope within the Antigenic Precursor Determines Immunogenicity and Protection against the Toxoplasma gondii Parasite Journal Article In: PLoS pathogens, vol. 9, no. 6, pp. e1003449, 2013, (Feliu, Virginie
Vasseur, Virginie
Grover, Harshita S
Chu, H Hamlet
Brown, Mark J
Wang, Jeremy
Boyle, Jon P
Robey, Ellen A
Shastri, Nilabh
Blanchard, Nicolas
PLoS Pathog. 2013 Jun;9(6):e1003449. doi: 10.1371/journal.ppat.1003449. Epub 2013 Jun 20.). @article{b,
title = {Location of the CD8 T Cell Epitope within the Antigenic Precursor Determines Immunogenicity and Protection against the Toxoplasma gondii Parasite},
author = {Feliu, V. and Vasseur, V. and Grover, H. S. and Chu, H. H. and Brown, M. J. and Wang, J. and Boyle, J. P. and Robey, E. A. and Shastri, N. and Blanchard, N.},
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abstract = {CD8 T cells protect the host from disease caused by intracellular pathogens, such as the Toxoplasma gondii (T. gondii) protozoan parasite. Despite the complexity of the T. gondii proteome, CD8 T cell responses are restricted to only a small number of peptide epitopes derived from a limited set of antigenic precursors. This phenomenon is known as immunodominance and is key to effective vaccine design. However, the mechanisms that determine the immunogenicity and immunodominance hierarchy of parasite antigens are not well understood. Here, using genetically modified parasites, we show that parasite burden is controlled by the immunodominant GRA6-specific CD8 T cell response but not by responses to the subdominant GRA4- and ROP7-derived epitopes. Remarkably, optimal processing and immunodominance were determined by the location of the peptide epitope at the C-terminus of the GRA6 antigenic precursor. In contrast, immunodominance could not be explained by the peptide affinity for the MHC I molecule or the frequency of T cell precursors in the naive animals. Our results reveal the molecular requirements for optimal presentation of an intracellular parasite antigen and for eliciting protective CD8 T cells.},
note = {Feliu, Virginie
Vasseur, Virginie
Grover, Harshita S
Chu, H Hamlet
Brown, Mark J
Wang, Jeremy
Boyle, Jon P
Robey, Ellen A
Shastri, Nilabh
Blanchard, Nicolas
PLoS Pathog. 2013 Jun;9(6):e1003449. doi: 10.1371/journal.ppat.1003449. Epub 2013 Jun 20.},
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CD8 T cells protect the host from disease caused by intracellular pathogens, such as the Toxoplasma gondii (T. gondii) protozoan parasite. Despite the complexity of the T. gondii proteome, CD8 T cell responses are restricted to only a small number of peptide epitopes derived from a limited set of antigenic precursors. This phenomenon is known as immunodominance and is key to effective vaccine design. However, the mechanisms that determine the immunogenicity and immunodominance hierarchy of parasite antigens are not well understood. Here, using genetically modified parasites, we show that parasite burden is controlled by the immunodominant GRA6-specific CD8 T cell response but not by responses to the subdominant GRA4- and ROP7-derived epitopes. Remarkably, optimal processing and immunodominance were determined by the location of the peptide epitope at the C-terminus of the GRA6 antigenic precursor. In contrast, immunodominance could not be explained by the peptide affinity for the MHC I molecule or the frequency of T cell precursors in the naive animals. Our results reveal the molecular requirements for optimal presentation of an intracellular parasite antigen and for eliciting protective CD8 T cells. |
Witkowski, B.; Khim, N.; Chim, P.; Kim, S.; Ke, S.; Kloeung, N.; Chy, S.; Duong, S.; Leang, R.; Ringwald, P.; Dondorp, A. M.; Tripura, R.; Benoit-Vical, F.; Berry, A.; Gorgette, O.; Ariey, F.; Barale, J. C.; Mercereau-Puijalon, O.; Menard, D. Reduced artemisinin susceptibility of Plasmodium falciparum ring stages in western Cambodia Journal Article In: Antimicrob Agents Chemother, vol. 57, no. 2, pp. 914-23, 2013, (Witkowski, Benoit
Khim, Nimol
Chim, Pheaktra
Kim, Saorin
Ke, Sopheakvatey
Kloeung, Nimol
Chy, Sophy
Duong, Socheat
Leang, Rithea
Ringwald, Pascal
Dondorp, Arjen M
Tripura, Rupam
Benoit-Vical, Francoise
Berry, Antoine
Gorgette, Olivier
Ariey, Frederic
Barale, Jean-Christophe
Mercereau-Puijalon, Odile
Menard, Didier
Antimicrob Agents Chemother. 2013 Feb;57(2):914-23. doi: 10.1128/AAC.01868-12. Epub 2012 Dec 3.). @article{b,
title = {Reduced artemisinin susceptibility of Plasmodium falciparum ring stages in western Cambodia},
author = {Witkowski, B. and Khim, N. and Chim, P. and Kim, S. and Ke, S. and Kloeung, N. and Chy, S. and Duong, S. and Leang, R. and Ringwald, P. and Dondorp, A. M. and Tripura, R. and Benoit-Vical, F. and Berry, A. and Gorgette, O. and Ariey, F. and Barale, J. C. and Mercereau-Puijalon, O. and Menard, D.},
year = {2013},
date = {2013-01-01},
journal = {Antimicrob Agents Chemother},
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abstract = {The declining efficacy of artemisinin derivatives against Plasmodium falciparum in western Cambodia is a major concern. The knowledge gap in the understanding of the mechanisms involved hampers designing monitoring tools. Here, we culture-adapted 20 isolates from Pailin and Ratanakiri (areas of artemisinin resistance and susceptibility in western and eastern Cambodia, respectively) and studied their in vitro response to dihydroartemisinin. No significant difference between the two sets of isolates was observed in the classical isotopic test. However, a 6-h pulse exposure to 700 nM dihydroartemisinin (ring-stage survival assay -RSA]) revealed a clear-cut geographic dichotomy. The survival rate of exposed ring-stage parasites (ring stages) was 17-fold higher in isolates from Pailin (median, 13.5%) than in those from Ratanakiri (median, 0.8%), while exposed mature stages were equally and highly susceptible (0.6% and 0.7%, respectively). Ring stages survived drug exposure by cell cycle arrest and resumed growth upon drug withdrawal. The reduced susceptibility to artemisinin in Pailin appears to be associated with an altered in vitro phenotype of ring stages from Pailin in the RSA.},
note = {Witkowski, Benoit
Khim, Nimol
Chim, Pheaktra
Kim, Saorin
Ke, Sopheakvatey
Kloeung, Nimol
Chy, Sophy
Duong, Socheat
Leang, Rithea
Ringwald, Pascal
Dondorp, Arjen M
Tripura, Rupam
Benoit-Vical, Francoise
Berry, Antoine
Gorgette, Olivier
Ariey, Frederic
Barale, Jean-Christophe
Mercereau-Puijalon, Odile
Menard, Didier
Antimicrob Agents Chemother. 2013 Feb;57(2):914-23. doi: 10.1128/AAC.01868-12. Epub 2012 Dec 3.},
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The declining efficacy of artemisinin derivatives against Plasmodium falciparum in western Cambodia is a major concern. The knowledge gap in the understanding of the mechanisms involved hampers designing monitoring tools. Here, we culture-adapted 20 isolates from Pailin and Ratanakiri (areas of artemisinin resistance and susceptibility in western and eastern Cambodia, respectively) and studied their in vitro response to dihydroartemisinin. No significant difference between the two sets of isolates was observed in the classical isotopic test. However, a 6-h pulse exposure to 700 nM dihydroartemisinin (ring-stage survival assay -RSA]) revealed a clear-cut geographic dichotomy. The survival rate of exposed ring-stage parasites (ring stages) was 17-fold higher in isolates from Pailin (median, 13.5%) than in those from Ratanakiri (median, 0.8%), while exposed mature stages were equally and highly susceptible (0.6% and 0.7%, respectively). Ring stages survived drug exposure by cell cycle arrest and resumed growth upon drug withdrawal. The reduced susceptibility to artemisinin in Pailin appears to be associated with an altered in vitro phenotype of ring stages from Pailin in the RSA. |
Ariey, F.; Witkowski, B.; Amaratunga, C.; Beghain, J.; Langlois, A. C.; Khim, N.; Kim, S.; Duru, V.; Bouchier, C.; Ma, L.; Lim, P.; Leang, R.; Duong, S.; Sreng, S.; Suon, S.; Chuor, C. M.; Bout, D. M.; Menard, S.; Rogers, W. O.; Genton, B.; Fandeur, T.; Miotto, O.; Ringwald, P.; Le Bras, J.; Berry, A.; Barale, J. C.; Fairhurst, R. M.; Benoit-Vical, F.; Mercereau-Puijalon, O.; Menard, D. A molecular marker of artemisinin-resistant Plasmodium falciparum malaria Journal Article In: Nature, 2013, (Dec 18. doi: 10.1038/nature12876.). @article{,
title = {A molecular marker of artemisinin-resistant Plasmodium falciparum malaria},
author = {Ariey, F. and Witkowski, B. and Amaratunga, C. and Beghain, J. and Langlois, A. C. and Khim, N. and Kim, S. and Duru, V. and Bouchier, C. and Ma, L. and Lim, P. and Leang, R. and Duong, S. and Sreng, S. and Suon, S. and Chuor, C. M. and Bout, D. M. and Menard, S. and Rogers, W. O. and Genton, B. and Fandeur, T. and Miotto, O. and Ringwald, P. and Le Bras, J. and Berry, A. and Barale, J. C. and Fairhurst, R. M. and Benoit-Vical, F. and Mercereau-Puijalon, O. and Menard, D.},
year = {2013},
date = {2013-01-01},
journal = {Nature},
abstract = {Plasmodium falciparum resistance to artemisinin derivatives in southeast Asia threatens malaria control and elimination activities worldwide. To monitor the spread of artemisinin resistance, a molecular marker is urgently needed. Here, using whole-genome sequencing of an artemisinin-resistant parasite line from Africa and clinical parasite isolates from Cambodia, we associate mutations in the PF3D7_1343700 kelch propeller domain ('K13-propeller') with artemisinin resistance in vitro and in vivo. Mutant K13-propeller alleles cluster in Cambodian provinces where resistance is prevalent, and the increasing frequency of a dominant mutant K13-propeller allele correlates with the recent spread of resistance in western Cambodia. Strong correlations between the presence of a mutant allele, in vitro parasite survival rates and in vivo parasite clearance rates indicate that K13-propeller mutations are important determinants of artemisinin resistance. K13-propeller polymorphism constitutes a useful molecular marker for large-scale surveillance efforts to contain artemisinin resistance in the Greater Mekong Subregion and prevent its global spread.},
note = {Dec 18. doi: 10.1038/nature12876.},
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Plasmodium falciparum resistance to artemisinin derivatives in southeast Asia threatens malaria control and elimination activities worldwide. To monitor the spread of artemisinin resistance, a molecular marker is urgently needed. Here, using whole-genome sequencing of an artemisinin-resistant parasite line from Africa and clinical parasite isolates from Cambodia, we associate mutations in the PF3D7_1343700 kelch propeller domain ('K13-propeller') with artemisinin resistance in vitro and in vivo. Mutant K13-propeller alleles cluster in Cambodian provinces where resistance is prevalent, and the increasing frequency of a dominant mutant K13-propeller allele correlates with the recent spread of resistance in western Cambodia. Strong correlations between the presence of a mutant allele, in vitro parasite survival rates and in vivo parasite clearance rates indicate that K13-propeller mutations are important determinants of artemisinin resistance. K13-propeller polymorphism constitutes a useful molecular marker for large-scale surveillance efforts to contain artemisinin resistance in the Greater Mekong Subregion and prevent its global spread. |
Feliu, V.; Vasseur, V.; Grover, H. S.; Chu, H. H.; Brown, M. J.; Wang, J.; Boyle, J. P.; Robey, E. A.; Shastri, N.; Blanchard, N. Location of the CD8 T Cell Epitope within the Antigenic Precursor Determines Immunogenicity and Protection against the Toxoplasma gondii Parasite Journal Article In: PLoS pathogens, vol. 9, no. 6, pp. e1003449, 2013, (Jun;9(6):e1003449. doi: 10.1371/journal.ppat.1003449. Epub 2013 Jun 20.). @article{b,
title = {Location of the CD8 T Cell Epitope within the Antigenic Precursor Determines Immunogenicity and Protection against the Toxoplasma gondii Parasite},
author = {Feliu, V. and Vasseur, V. and Grover, H. S. and Chu, H. H. and Brown, M. J. and Wang, J. and Boyle, J. P. and Robey, E. A. and Shastri, N. and Blanchard, N.},
year = {2013},
date = {2013-01-01},
journal = {PLoS pathogens},
volume = {9},
number = {6},
pages = {e1003449},
abstract = {CD8 T cells protect the host from disease caused by intracellular pathogens, such as the Toxoplasma gondii (T. gondii) protozoan parasite. Despite the complexity of the T. gondii proteome, CD8 T cell responses are restricted to only a small number of peptide epitopes derived from a limited set of antigenic precursors. This phenomenon is known as immunodominance and is key to effective vaccine design. However, the mechanisms that determine the immunogenicity and immunodominance hierarchy of parasite antigens are not well understood. Here, using genetically modified parasites, we show that parasite burden is controlled by the immunodominant GRA6-specific CD8 T cell response but not by responses to the subdominant GRA4- and ROP7-derived epitopes. Remarkably, optimal processing and immunodominance were determined by the location of the peptide epitope at the C-terminus of the GRA6 antigenic precursor. In contrast, immunodominance could not be explained by the peptide affinity for the MHC I molecule or the frequency of T cell precursors in the naive animals. Our results reveal the molecular requirements for optimal presentation of an intracellular parasite antigen and for eliciting protective CD8 T cells.},
note = {Jun;9(6):e1003449. doi: 10.1371/journal.ppat.1003449. Epub 2013 Jun 20.},
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CD8 T cells protect the host from disease caused by intracellular pathogens, such as the Toxoplasma gondii (T. gondii) protozoan parasite. Despite the complexity of the T. gondii proteome, CD8 T cell responses are restricted to only a small number of peptide epitopes derived from a limited set of antigenic precursors. This phenomenon is known as immunodominance and is key to effective vaccine design. However, the mechanisms that determine the immunogenicity and immunodominance hierarchy of parasite antigens are not well understood. Here, using genetically modified parasites, we show that parasite burden is controlled by the immunodominant GRA6-specific CD8 T cell response but not by responses to the subdominant GRA4- and ROP7-derived epitopes. Remarkably, optimal processing and immunodominance were determined by the location of the peptide epitope at the C-terminus of the GRA6 antigenic precursor. In contrast, immunodominance could not be explained by the peptide affinity for the MHC I molecule or the frequency of T cell precursors in the naive animals. Our results reveal the molecular requirements for optimal presentation of an intracellular parasite antigen and for eliciting protective CD8 T cells. |
Witkowski, B.; Khim, N.; Chim, P.; Kim, S.; Ke, S.; Kloeung, N.; Chy, S.; Duong, S.; Leang, R.; Ringwald, P.; Dondorp, A. M.; Tripura, R.; Benoit-Vical, F.; Berry, A.; Gorgette, O.; Ariey, F.; Barale, J. C.; Mercereau-Puijalon, O.; Menard, D. Reduced artemisinin susceptibility of Plasmodium falciparum ring stages in western Cambodia Journal Article In: Antimicrob Agents Chemother, vol. 57, no. 2, pp. 914-23, 2013, (Feb;57(2):914-23. doi: 10.1128/AAC.01868-12. Epub 2012 Dec 3.). @article{b,
title = {Reduced artemisinin susceptibility of Plasmodium falciparum ring stages in western Cambodia},
author = {Witkowski, B. and Khim, N. and Chim, P. and Kim, S. and Ke, S. and Kloeung, N. and Chy, S. and Duong, S. and Leang, R. and Ringwald, P. and Dondorp, A. M. and Tripura, R. and Benoit-Vical, F. and Berry, A. and Gorgette, O. and Ariey, F. and Barale, J. C. and Mercereau-Puijalon, O. and Menard, D.},
year = {2013},
date = {2013-01-01},
journal = {Antimicrob Agents Chemother},
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number = {2},
pages = {914-23},
abstract = {The declining efficacy of artemisinin derivatives against Plasmodium falciparum in western Cambodia is a major concern. The knowledge gap in the understanding of the mechanisms involved hampers designing monitoring tools. Here, we culture-adapted 20 isolates from Pailin and Ratanakiri (areas of artemisinin resistance and susceptibility in western and eastern Cambodia, respectively) and studied their in vitro response to dihydroartemisinin. No significant difference between the two sets of isolates was observed in the classical isotopic test. However, a 6-h pulse exposure to 700 nM dihydroartemisinin (ring-stage survival assay -RSA]) revealed a clear-cut geographic dichotomy. The survival rate of exposed ring-stage parasites (ring stages) was 17-fold higher in isolates from Pailin (median, 13.5%) than in those from Ratanakiri (median, 0.8%), while exposed mature stages were equally and highly susceptible (0.6% and 0.7%, respectively). Ring stages survived drug exposure by cell cycle arrest and resumed growth upon drug withdrawal. The reduced susceptibility to artemisinin in Pailin appears to be associated with an altered in vitro phenotype of ring stages from Pailin in the RSA.},
note = {Feb;57(2):914-23. doi: 10.1128/AAC.01868-12. Epub 2012 Dec 3.},
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The declining efficacy of artemisinin derivatives against Plasmodium falciparum in western Cambodia is a major concern. The knowledge gap in the understanding of the mechanisms involved hampers designing monitoring tools. Here, we culture-adapted 20 isolates from Pailin and Ratanakiri (areas of artemisinin resistance and susceptibility in western and eastern Cambodia, respectively) and studied their in vitro response to dihydroartemisinin. No significant difference between the two sets of isolates was observed in the classical isotopic test. However, a 6-h pulse exposure to 700 nM dihydroartemisinin (ring-stage survival assay -RSA]) revealed a clear-cut geographic dichotomy. The survival rate of exposed ring-stage parasites (ring stages) was 17-fold higher in isolates from Pailin (median, 13.5%) than in those from Ratanakiri (median, 0.8%), while exposed mature stages were equally and highly susceptible (0.6% and 0.7%, respectively). Ring stages survived drug exposure by cell cycle arrest and resumed growth upon drug withdrawal. The reduced susceptibility to artemisinin in Pailin appears to be associated with an altered in vitro phenotype of ring stages from Pailin in the RSA. |
Szelechowski, Marion; Bergeron, Corinne; Gonzalez-Dunia, Daniel; Klonjkowski, Bernard Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors Journal Article In: JoVE, no. 82, 2013, ISSN: 1940-087X. @article{Szelechowski2013,
title = {Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors},
author = {Marion Szelechowski and Corinne Bergeron and Daniel Gonzalez-Dunia and Bernard Klonjkowski},
doi = {10.3791/50833},
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de Koning, H D; van den Bogaard, E H; Bergboer, J G M; Kamsteeg, M; van Vlijmen-Willems, I M J J; Hitomi, K; Henry, J; Simon, M; Takashita, N; Ishida-Yamamoto, A; Schalkwijk, J; Zeeuwen, P L J M Expression profile of cornified envelope structural proteins and keratinocyte differentiation-regulating proteins during skin barrier repair. Journal Article In: The British journal of dermatology, vol. 166, no. 6, pp. 1245–1254, 2012, ISSN: 1365-2133 (Electronic). @article{DeKoning2012,
title = {Expression profile of cornified envelope structural proteins and keratinocyte differentiation-regulating proteins during skin barrier repair.},
author = {de Koning, H D and van den Bogaard, E H and Bergboer, J G M and Kamsteeg, M and van Vlijmen-Willems, I M J J and Hitomi, K and Henry, J and Simon, M and Takashita, N and Ishida-Yamamoto, A and Schalkwijk, J and Zeeuwen, P L J M},
doi = {10.1111/j.1365-2133.2012.10885.x},
issn = {1365-2133 (Electronic)},
year = {2012},
date = {2012-06-01},
journal = {The British journal of dermatology},
volume = {166},
number = {6},
pages = {1245--1254},
abstract = {BACKGROUND: Recent studies have emphasized the importance of heritable and acquired skin barrier abnormalities in common inflammatory diseases such as psoriasis and atopic dermatitis (AD). To date, no comprehensive studies on the effect of experimental barrier disruption on cornified envelope protein expression have been performed. OBJECTIVES: To analyse the effect of experimental skin barrier disruption on the expression of cornified envelope structural proteins and keratinocyte differentiation-regulating proteins. METHODS: We examined mRNA (day 1, 3 and 7) and protein (day 1, 2, 4 and 9) expression levels of structural proteins and regulatory molecules after sodium dodecyl sulphate (SDS) application on normal skin, and tape stripping of uninvolved epidermis of patients with psoriasis and AD and healthy controls. RESULTS: Upon tape stripping, several structural molecules were significantly downregulated (at the mRNA level as well as the protein level), including LCE5A, LCE2B, FLG, FLG2 and LOR, whereas others were upregulated: IVL, SPRR1, SPRR2, HRNR and most notably LCE3A. The epidermal crosslinking enzymes TGM1, TGM3 and TGM5 were all upregulated, whereas proteases involved in the desquamation process (CTSV, KLK5 and KLK7) were downregulated or unaffected. Most results were similar in SDS-instigated irritant contact dermatitis. There was no significant difference in response between normal epidermis and nonlesional skin of patients with psoriasis and AD. CONCLUSIONS: Skin barrier disruption induces a temporary barrier repair response composed of increased expression of several cornification-related proteins, and decreased expression of some structural and desquamation-related proteins.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
BACKGROUND: Recent studies have emphasized the importance of heritable and acquired skin barrier abnormalities in common inflammatory diseases such as psoriasis and atopic dermatitis (AD). To date, no comprehensive studies on the effect of experimental barrier disruption on cornified envelope protein expression have been performed. OBJECTIVES: To analyse the effect of experimental skin barrier disruption on the expression of cornified envelope structural proteins and keratinocyte differentiation-regulating proteins. METHODS: We examined mRNA (day 1, 3 and 7) and protein (day 1, 2, 4 and 9) expression levels of structural proteins and regulatory molecules after sodium dodecyl sulphate (SDS) application on normal skin, and tape stripping of uninvolved epidermis of patients with psoriasis and AD and healthy controls. RESULTS: Upon tape stripping, several structural molecules were significantly downregulated (at the mRNA level as well as the protein level), including LCE5A, LCE2B, FLG, FLG2 and LOR, whereas others were upregulated: IVL, SPRR1, SPRR2, HRNR and most notably LCE3A. The epidermal crosslinking enzymes TGM1, TGM3 and TGM5 were all upregulated, whereas proteases involved in the desquamation process (CTSV, KLK5 and KLK7) were downregulated or unaffected. Most results were similar in SDS-instigated irritant contact dermatitis. There was no significant difference in response between normal epidermis and nonlesional skin of patients with psoriasis and AD. CONCLUSIONS: Skin barrier disruption induces a temporary barrier repair response composed of increased expression of several cornification-related proteins, and decreased expression of some structural and desquamation-related proteins. |
Lagarrigue, Sophie Garrido; George, Jerome; Questel, Emmanuel; Lauze, Christophe; Meyer, Nicolas; Lagarde, Jean-Michel; Simon, Michel; Schmitt, Anne-Marie; Serre, Guy; Paul, Carle In vivo quantification of epidermis pigmentation and dermis papilla density with reflectance confocal microscopy: variations with age and skin phototype. Journal Article In: Experimental dermatology, vol. 21, no. 4, pp. 281–286, 2012, ISSN: 1600-0625 (Electronic). @article{Lagarrigue2012,
title = {In vivo quantification of epidermis pigmentation and dermis papilla density with reflectance confocal microscopy: variations with age and skin phototype.},
author = {Lagarrigue, Sophie Garrido and George, Jerome and Questel, Emmanuel and Lauze, Christophe and Meyer, Nicolas and Lagarde, Jean-Michel and Simon, Michel and Schmitt, Anne-Marie and Serre, Guy and Paul, Carle},
doi = {10.1111/j.1600-0625.2012.01451.x},
issn = {1600-0625 (Electronic)},
year = {2012},
date = {2012-04-01},
journal = {Experimental dermatology},
volume = {21},
number = {4},
pages = {281--286},
abstract = {Reflectance confocal microscopy (RCM) may help to quantify variations of skin pigmentation induced by different stimuli such as UV radiation or therapeutic intervention. The objective of our work was to identify RCM parameters able to quantify in vivo dermis papilla density and epidermis pigmentation potentially applicable in clinical studies. The study included 111 healthy female volunteers with phototypes I-VI. Photo-exposed and photo-protected anatomical sites were imaged. The effect of age was also assessed. Four epidermis components were specifically investigated: stratum corneum, stratum spinosum, basal epidermal layer and dermo-epidermal junction. Laser power, diameter of corneocytes and upper spinous keratinocytes, brightness of upper spinous and interpapillary spinous keratinocytes, number of dermal papillae and papillary contrast were systematically assessed. Papillary contrast measured at the dermo-epidermal junction appeared to be a reliable marker of epidermis pigmentation and showed a strong correlation with skin pigmentation assessed clinically using the Fitzpatrick's classification. Brightness of upper spinous and interpapillary spinous keratinocytes was not influenced by the skin phototype. The number of dermal papillae was significantly lower in subjects with phototypes I-II as compared with darker skin subjects. A dramatic reduction in the number of dermal papillae was noticed with age, particularly in subjects with fair skin. The method presented here provides a new in vivo investigation tool for quantification of dermis papilla density and epidermal pigmentation. Papillary contrast measured at the dermo-epidermal junction may be selected as a marker of skin pigmentation for evaluation in clinical studies.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Reflectance confocal microscopy (RCM) may help to quantify variations of skin pigmentation induced by different stimuli such as UV radiation or therapeutic intervention. The objective of our work was to identify RCM parameters able to quantify in vivo dermis papilla density and epidermis pigmentation potentially applicable in clinical studies. The study included 111 healthy female volunteers with phototypes I-VI. Photo-exposed and photo-protected anatomical sites were imaged. The effect of age was also assessed. Four epidermis components were specifically investigated: stratum corneum, stratum spinosum, basal epidermal layer and dermo-epidermal junction. Laser power, diameter of corneocytes and upper spinous keratinocytes, brightness of upper spinous and interpapillary spinous keratinocytes, number of dermal papillae and papillary contrast were systematically assessed. Papillary contrast measured at the dermo-epidermal junction appeared to be a reliable marker of epidermis pigmentation and showed a strong correlation with skin pigmentation assessed clinically using the Fitzpatrick's classification. Brightness of upper spinous and interpapillary spinous keratinocytes was not influenced by the skin phototype. The number of dermal papillae was significantly lower in subjects with phototypes I-II as compared with darker skin subjects. A dramatic reduction in the number of dermal papillae was noticed with age, particularly in subjects with fair skin. The method presented here provides a new in vivo investigation tool for quantification of dermis papilla density and epidermal pigmentation. Papillary contrast measured at the dermo-epidermal junction may be selected as a marker of skin pigmentation for evaluation in clinical studies. |
Mazereeuw-Hautier, J; Dreyfus, I; Barbarot, S; Serrentino, L; Bourdon-Lanoy, E; Ezzedine, K; Maza, A; Aujoulat, I; Le Rhun, A Factors influencing quality of life in patients with inherited ichthyosis: a qualitative study in adults using focus groups. Journal Article In: The British journal of dermatology, vol. 166, no. 3, pp. 646–648, 2012, ISSN: 1365-2133 (Electronic). @article{Mazereeuw-Hautier2012,
title = {Factors influencing quality of life in patients with inherited ichthyosis: a qualitative study in adults using focus groups.},
author = {Mazereeuw-Hautier, J and Dreyfus, I and Barbarot, S and Serrentino, L and Bourdon-Lanoy, E and Ezzedine, K and Maza, A and Aujoulat, I and Le Rhun, A},
doi = {10.1111/j.1365-2133.2011.10701.x},
issn = {1365-2133 (Electronic)},
year = {2012},
date = {2012-03-01},
journal = {The British journal of dermatology},
volume = {166},
number = {3},
pages = {646--648},
abstract = {BACKGROUND: There is limited information regarding quality of life in patients with inherited ichthyosis. OBJECTIVES: To identify factors influencing quality of life in patients with inherited ichthyosis. METHODS: The study used focus groups and involved adult patients suffering from inherited ichthyosis from three French hospital centres. Group discussions were conducted by two facilitators and were continued until data saturation was reached. The verbatim transcripts were analysed independently by two investigators. Categories considered as key factors in the modulation of quality of life were negotiated until agreement was obtained. RESULTS: Data saturation was reached after the fifth group. A total of 25 patients affected by various forms of ichthyosis attended these focus groups. The identified factors influencing quality of life were related to physical health, daily life, relations with others or oneself. However, together with difficulties related to ichthyosis, patients also underlined some positive aspects of the disease and described specific measures used to improve their quality of life. CONCLUSIONS: This is the first study investigating the different factors that could impact quality of life in patients with ichthyosis. This provides an essential framework from which physicians can develop strategies to improve patient care and quality of life and to develop a specific quality of life questionnaire.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
BACKGROUND: There is limited information regarding quality of life in patients with inherited ichthyosis. OBJECTIVES: To identify factors influencing quality of life in patients with inherited ichthyosis. METHODS: The study used focus groups and involved adult patients suffering from inherited ichthyosis from three French hospital centres. Group discussions were conducted by two facilitators and were continued until data saturation was reached. The verbatim transcripts were analysed independently by two investigators. Categories considered as key factors in the modulation of quality of life were negotiated until agreement was obtained. RESULTS: Data saturation was reached after the fifth group. A total of 25 patients affected by various forms of ichthyosis attended these focus groups. The identified factors influencing quality of life were related to physical health, daily life, relations with others or oneself. However, together with difficulties related to ichthyosis, patients also underlined some positive aspects of the disease and described specific measures used to improve their quality of life. CONCLUSIONS: This is the first study investigating the different factors that could impact quality of life in patients with ichthyosis. This provides an essential framework from which physicians can develop strategies to improve patient care and quality of life and to develop a specific quality of life questionnaire. |
De Rocca Serra-Nedelec, A.; Edouard, T.; Treguer, K.; Tajan, M.; Araki, T.; Dance, M.; Mus, M.; Montagner, A.; Tauber, M.; Salles, J. P.; Valet, P.; Neel, B. G.; Raynal, P.; Yart, A. Noonan syndrome-causing SHP2 mutants inhibit insulin-like growth factor 1 release via growth hormone-induced ERK hyperactivation, which contributes to short stature Journal Article In: Proc Natl Acad Sci U S A, vol. 109, no. 11, pp. 4257-62, 2012, ISSN: 1091-6490 (Electronic)
0027-8424 (Linking). @article{RN16b,
title = {Noonan syndrome-causing SHP2 mutants inhibit insulin-like growth factor 1 release via growth hormone-induced ERK hyperactivation, which contributes to short stature},
author = {De Rocca Serra-Nedelec, A. and Edouard, T. and Treguer, K. and Tajan, M. and Araki, T. and Dance, M. and Mus, M. and Montagner, A. and Tauber, M. and Salles, J. P. and Valet, P. and Neel, B. G. and Raynal, P. and Yart, A.},
url = {https://www.ncbi.nlm.nih.gov/pubmed/22371576},
doi = {10.1073/pnas.1119803109},
issn = {1091-6490 (Electronic)
0027-8424 (Linking)},
year = {2012},
date = {2012-01-01},
journal = {Proc Natl Acad Sci U S A},
volume = {109},
number = {11},
pages = {4257-62},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Boyer, J. F.; Bongard, V.; Cantagrel, A.; Jamard, B.; Gottenberg, J. E.; Mariette, X.; Davignon, J. L.; Ferrieres, J.; Ruidavets, J. B.; Dallongeville, J.; Arveiler, D.; Cambon-Thomsen, A.; Constantin, A. Link between traditional cardiovascular risk factors and inflammation in patients with early arthritis: results from a French multicenter cohort Journal Article In: Arthritis Care Res (Hoboken), vol. 64, no. 6, pp. 872-80, 2012, ISSN: 2151-4658 (Electronic)
2151-464X (Linking). @article{RN12b,
title = {Link between traditional cardiovascular risk factors and inflammation in patients with early arthritis: results from a French multicenter cohort},
author = {Boyer, J. F. and Bongard, V. and Cantagrel, A. and Jamard, B. and Gottenberg, J. E. and Mariette, X. and Davignon, J. L. and Ferrieres, J. and Ruidavets, J. B. and Dallongeville, J. and Arveiler, D. and Cambon-Thomsen, A. and Constantin, A.},
url = {https://www.ncbi.nlm.nih.gov/pubmed/22275176},
doi = {10.1002/acr.21623},
issn = {2151-4658 (Electronic)
2151-464X (Linking)},
year = {2012},
date = {2012-01-01},
journal = {Arthritis Care Res (Hoboken)},
volume = {64},
number = {6},
pages = {872-80},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Guerder, S.; Viret, C.; Luche, H.; Ardouin, L.; Malissen, B. Differential processing of self-antigens by subsets of thymic stromal cells Journal Article In: Curr Opin Immunol, vol. 24, no. 1, pp. 99-104, 2012, ISSN: 1879-0372 (Electronic)
0952-7915 (Linking). @article{RN12,
title = {Differential processing of self-antigens by subsets of thymic stromal cells},
author = {Guerder, S. and Viret, C. and Luche, H. and Ardouin, L. and Malissen, B.},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22296716},
doi = {10.1016/j.coi.2012.01.008},
issn = {1879-0372 (Electronic)
0952-7915 (Linking)},
year = {2012},
date = {2012-01-01},
journal = {Curr Opin Immunol},
volume = {24},
number = {1},
pages = {99-104},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Romagnoli, P.; Dooley, J.; Enault, G.; Vicente, R.; Malissen, B.; Liston, A.; van Meerwijk, J. P. M. The thymic niche does not limit development of the naturally diverse population of mouse regulatory T lymphocytes Journal Article In: Journal of Immunology, vol. 189, no. 8, pp. 3831-7, 2012, ISSN: 1550-6606 (Electronic)
0022-1767 (Linking). @article{RN166,
title = {The thymic niche does not limit development of the naturally diverse population of mouse regulatory T lymphocytes},
author = {Romagnoli, P. and Dooley, J. and Enault, G. and Vicente, R. and Malissen, B. and Liston, A. and van Meerwijk, J. P.M.},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22988035},
doi = {10.4049/jimmunol.1201564},
issn = {1550-6606 (Electronic)
0022-1767 (Linking)},
year = {2012},
date = {2012-01-01},
journal = {Journal of Immunology},
volume = {189},
number = {8},
pages = {3831-7},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Boue, J.; Blanpied, C.; Djata-Cabral, M.; Pelletier, L.; Vergnolle, N.; Dietrich, G. Immune conditions associated with CD4+ T effector-induced opioid release and analgesia Journal Article In: Pain, vol. 153, no. 2, pp. 485-93, 2012, ISSN: 1872-6623 (Electronic)
0304-3959 (Linking). @article{RN18,
title = {Immune conditions associated with CD4+ T effector-induced opioid release and analgesia},
author = {Boue, J. and Blanpied, C. and Djata-Cabral, M. and Pelletier, L. and Vergnolle, N. and Dietrich, G.},
url = {https://www.ncbi.nlm.nih.gov/pubmed/22188867},
doi = {10.1016/j.pain.2011.11.013},
issn = {1872-6623 (Electronic)
0304-3959 (Linking)},
year = {2012},
date = {2012-01-01},
journal = {Pain},
volume = {153},
number = {2},
pages = {485-93},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Guery, J. C. Estrogens and inflammatory autoimmune diseases Journal Article In: Joint, bone, spine : revue du rhumatisme, vol. 79, no. 6, pp. 560-2, 2012, ISSN: 1778-7254 (Electronic)
1297-319X (Linking). @article{RN2b,
title = {Estrogens and inflammatory autoimmune diseases},
author = {Guery, J. C.},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23141100},
doi = {10.1016/j.jbspin.2012.09.010},
issn = {1778-7254 (Electronic)
1297-319X (Linking)},
year = {2012},
date = {2012-01-01},
journal = {Joint, bone, spine : revue du rhumatisme},
volume = {79},
number = {6},
pages = {560-2},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Pendaries, V.; Gasc, G.; Titeux, M.; Tonasso, L.; Mejia, J. E.; Hovnanian, A. siRNA-mediated allele-specific inhibition of mutant type VII collagen in dominant dystrophic epidermolysis bullosa Journal Article In: J Invest Dermatol, vol. 132, no. 6, pp. 1741-3, 2012, ISSN: 1523-1747 (Electronic)
0022-202X (Linking). @article{RN17b,
title = {siRNA-mediated allele-specific inhibition of mutant type VII collagen in dominant dystrophic epidermolysis bullosa},
author = {Pendaries, V. and Gasc, G. and Titeux, M. and Tonasso, L. and Mejia, J. E. and Hovnanian, A.},
url = {https://www.ncbi.nlm.nih.gov/pubmed/22336946},
doi = {10.1038/jid.2012.11},
issn = {1523-1747 (Electronic)
0022-202X (Linking)},
year = {2012},
date = {2012-01-01},
journal = {J Invest Dermatol},
volume = {132},
number = {6},
pages = {1741-3},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|